Does exposure of human skin in situ to 385 or 405 nm UV induce pyrimidine dimers in DNA?
Does exposure of human skin in situ to 385 or 405 nm UV induce pyrimidine dimers in DNA?
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人体皮肤原位暴露于 385 或 405 nm 紫外线下是否会诱导 DNA 中产生嘧啶二聚体？
DOI：
10.1111/j.1751-1097.1990.tb08698.x
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发表时间：
1990
影响因子：
3.3
通讯作者：
Sutherland,BM
中科院分区：
文献类型：
--
作者：
Hacham,H;Freeman,SE;Gange,RW;Maytum,DJ;Sutherland,JC;Sutherland,BM
前一份报告[弗里曼特尔。（1986）光化学。Photobiol.43S，93S]表明，用385或405 nm辐射照射人体皮肤原位，在DNA中产生可检测水平的嘧啶二聚体。由于这些波长被DNA吸收很差，这些结果表明DNA损伤被皮肤中存在的其他吸收分子敏化。对先前工作的两个实验方面的检查表明：（1）用于损伤测定的DNA分散的静态凝胶电泳法给出了诱导的二聚体水平的准确值，和（2）由385 nm明显诱导的DNA损伤实际上是由单色仪的20 nm带通光束中存在的较短波长的UV诱导的。目前的结果表明，单色385和405 nm的辐射是无效的二聚体在人体皮肤原位生产。
A previous report [Freemanetal.(1986)Photochem. Photobiol.43S, 93S] indicated that irradiation of human skinin situwith 385 or 405 nm radiation produced detectable levels of pyrimidine dimers in DNA. Since these wavelengths are absorbed poorly by DNA, these results suggested that DNA damage was sensitized by other absorbing molecules present in skin. Examination of two experimental aspects of the previous work indicates that (1) the static gel electrophoresis method for DNA dispersion used in lesion determination gave accurate values of the levels of induced dimers, and (2) the DNA damage apparently induced by 385 nm was actually induced by shorter wavelength UV present in the 20 nm bandpass beam of the monochromator. The current results indicate that monochromatic 385 and 405 nm radiation are ineffective in dimer production in human skinin situ.