Blue Light- and Low Temperature-Regulated COR27 and COR28 Play Roles in the Arabidopsis Circadian Clock
Blue Light- and Low Temperature-Regulated COR27 and COR28 Play Roles in the Arabidopsis Circadian Clock
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蓝光和低温调节的 COR27 和 COR28 在拟南芥生物钟中发挥作用。
DOI：
10.1105/tpc.16.00354
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发表时间：
2016-11-01
期刊：
影响因子：
11.6
通讯作者：
Liu, Hongtao
中科院分区：
文献类型：
--
作者：
Li, Xu;Ma, Dingbang;Liu, Hongtao
光和温度是深刻影响植物生长发育的两个关键环境信号，但光和温度信号如何影响生物钟的潜在分子机制在很大程度上是未知的。在这里，我们报道了COR27和COR28不仅受到低温的调节，还受到光信号的调节。COR27和COR28是抗冻性的负调控因子，而对开花具有正调控作用，可能代表了抗冻性与开花之间的权衡关系。此外，COR27和COR28的功能缺失突变导致各种昼夜输出节律的周期延长，并影响中枢时钟基因的表达。此外，cor27 cor28双突变影响生物钟的节奏。此外，COR27和COR28是CCA1的直接靶点，CCA1通过染色质结合抑制它们的转录。最后，我们报道了COR27和COR28结合TOC1和PRR5的染色质抑制它们的转录，这表明它们对节律的影响部分是由于它们对TOC1和PRR5的调节。这些数据表明，蓝光和低温调节的COR27和COR28调节生物钟、抗冻性和开花时间。
Light and temperature are two key environmental signals that profoundly affect plant growth and development, but underlying molecular mechanisms of how light and temperature signals affect the circadian clock are largely unknown. Here, we report that COR27 and COR28 are regulated not only by low temperatures but also by light signals. COR27 and COR28 are negative regulators of freezing tolerance but positive regulators of flowering, possibly representing a trade-off between freezing tolerance and flowering. Furthermore, loss-of-function mutations in COR27 and COR28 result in period lengthening of various circadian output rhythms and affect central clock gene expression. Also, the cor27 cor28 double mutation affects the pace of the circadian clock. Additionally, COR27 and COR28 are direct targets of CCA1, which represses their transcription via chromatin binding. Finally, we report that COR27 and COR28 bind to the chromatin of TOC1 and PRR5 to repress their transcription, suggesting that their effects on rhythms are in part due to their regulation of TOC1 and PRR5. These data demonstrate that blue light and low temperature-regulated COR27 and COR28 regulate the circadian clock as well as freezing tolerance and flowering time.