Degradation of nonane by bacteria from Antarctic marine sediment
Degradation of nonane by bacteria from Antarctic marine sediment
复制标题
南极海洋沉积物中细菌降解壬烷
作者：
S. Powell;J. Bowman;I. Snape
从南极凯西站附近的沉积物中筛选出一种耐石油烃的微生物富集培养物。为了选择将降解柴油的冷适应微生物，在连续六次转移到新鲜培养基期间在0°C下培养富集物，其中特殊南极混合柴油（SAB）作为唯一碳源。然后在接种富集培养物的微宇宙中测量SAB组分的生物降解。16周后，生物降解的量很小，但壬烷（C9烷烃）的降解显着增加接种的缩影比无菌对照。然后从富集培养物中提取DNA，扩增16S rRNA基因片段用于变性梯度凝胶电泳。从凝胶上切下条带，测序后发现属于假单胞菌属和Colwellia属。
A microbial enrichment culture tolerant of petroleum hydrocarbons was developed from sediment collected near Casey Station, Antarctica. To select cold-adapted microbes that would degrade diesel, enrichments were cultured at 0°C during six successive transfers to fresh medium, with Special Antarctic Blend diesel (SAB) as the sole carbon source. Biodegradation of components of the SAB was then measured in microcosms inoculated with the enrichment culture. After 16 weeks, the amount of biodegradation was small, but nonane (a C9 alkane) had degraded significantly more in inoculated microcosms than in sterile controls. DNA was then extracted from the enrichment cultures and a fragment of the 16S rRNA gene was amplified for denaturing gradient gel electrophoresis. Bands were excised from the gel and, following sequencing, were found to belong to the genera Pseudomonas and Colwellia.