Characterization of Microbial Community in Membrane Bioreactors Treating Domestic Wastewater
Characterization of Microbial Community in Membrane Bioreactors Treating Domestic Wastewater
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DOI：
10.2965/jwet.2014.99
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发表时间：
2014
影响因子：
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通讯作者：
Kazuki Takada;Kurumi Hashimoto;S. Soda;M. Ike;K. Yamashita;T. Hashimoto
中科院分区：
文献类型：
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作者：
Kazuki Takada;Kurumi Hashimoto;S. Soda;M. Ike;K. Yamashita;T. Hashimoto
采用真细菌16 S rRNA基因末端限制性片段长度多态性（T-RFLP）分析和碳源利用试验（Biolog assay），对12个处理生活污水的膜生物反应器（MBR）活性污泥微生物群落的产酶组成和有机物代谢进行了研究。MBR污泥中的微生物结构在样品之间差异很大，既不取决于进水特性，也不取决于操作模式和条件。比较的微生物群落结构的12个MBR和11个传统的工艺，其特征在于在以前的研究表明，MBR和传统的工艺有很大的不同，在产碱结构方面。此外，MBR中的优势细菌彼此差异很大，尽管常规工艺中的优势细菌是相似的。相比之下，在12个MBR中的8个活性污泥样品的碳利用概况和在以前的研究中测试的所有常规工艺是相互类似的，表明活性污泥群落具有类似的生物降解潜力，对有机污染物，即使系统发育的细菌组成不同。结果还表明，一些MBR污泥样品具有独特的碳利用概况，这表明MBR丰富的细菌种群具有独特的代谢活性。
Activated sludge microbial communities of 12 membrane bioreactors (MBRs) treating domestic wastewater were characterized in terms of their bacterial phylogenic composition and organic metabolism using eubacterial 16S rRNA gene-based terminal-restriction fragment length polymorphism (T-RFLP) analysis and carbon-utilization tests (Biolog assay). The phylogenic microbial structure of MBR sludges differed considerably among samples, depending neither on influent characteristics nor on operational modes and conditions. Comparison of the microbial community structures of the 12 MBRs and 11 conventional processes characterized in a previous study indicated that MBRs and the conventional processes differ considerably in terms of phylogenic structure. Also, dominant bacteria in MBRs differed considerably from each other, although those in the conventional processes were similar. In contrast, the carbon-utilization profile of activated sludge samples of 8 among 12 MBRs and all conventional processes tested in the previous study were mutually similar , indicating that activated sludge communities possess similar biodegradation potential against organic pollutants even if the phylogenetic bacterial composition differs. Results also demonstrated that some MBR sludge samples have unique carbon-utilization profiles, suggesting that MBRs enrich bacterial populations with unique metabolic activity.