Enrichment of inflammatory mediators in the synovial fluid is associated with slower progression of mild to moderate osteoarthritis in the porcine knee.
Enrichment of inflammatory mediators in the synovial fluid is associated with slower progression of mild to moderate osteoarthritis in the porcine knee.
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发表时间：
2021
影响因子：
2.2
通讯作者：
N. Karamchedu;B. Fleming;J. Donnenfield;B. Proffen;Meggin Q Costa;J. Molino;M. Murray
中科院分区：
文献类型：
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作者：
N. Karamchedu;B. Fleming;J. Donnenfield;B. Proffen;Meggin Q Costa;J. Molino;M. Murray
细胞因子和基质金属蛋白酶在创伤后骨关节炎（PTOA）的发生和发展中的作用仍然是一个有争议的话题。研究目的是评价猪前交叉韧带（ACL）手术模型中轻度至中度PTOA发生期间这些炎症介质的浓度。我们假设，与发生轻度PTOA的猪（接受支架增强ACL修复的猪）相比，发生中度PTOA的猪（接受ACL重建或未治疗ACL横断的猪）中具有可检测介质的动物更多。36只尤卡坦小型猪接受了ACL横切，并随机分为：1）不进一步治疗，2）ACL重建，或3）支架增强ACL修复。在术前和术后1、4、12、26和52周采集滑液样本。通过多重测定评价滑液样品中炎性介质的浓度。在第52周人道处死后进行肉眼软骨评估。如先前的研究所发现的，修复组的软骨损伤明显少于ACL横断组或ACL重建组（P<0.03）。生物标志物的存在和浓度受手术组和时间的影响。总的来说，修复组的炎症介质浓度较高，其软骨损伤比其他两个治疗组少。虽然这一发现推翻了假设，但这些数据表明，在52周的时间内，软骨损伤较少的关节的代谢活动仍然高于那些没有软骨损伤的关节。
The roles that cytokines and matrix metalloproteinases play in the onset and progression of posttraumatic osteoarthritis (PTOA) remain a topic of debate. The study objective was to evaluate the concentrations of these inflammatory mediators during the development of mild to moderate PTOA in the porcine anterior cruciate ligament (ACL) surgical model. We hypothesized that there would be more animals with detectable mediators in the pigs that develop moderate PTOA (those receiving ACL reconstruction or untreated ACL transection) compared to those that develop mild PTOA (those receiving scaffold-enhanced ACL repair). 36 Yucatan minipigs underwent ACL transection and were randomized to: 1) no further treatment, 2) ACL reconstruction, or 3) scaffold-enhanced ACL repair. Synovial fluid samples were obtained pre-operatively, and at 1, 4, 12, 26 and 52 weeks post-operatively. The concentrations of inflammatory mediator in the synovial fluid samples were evaluated via multiplex assay. Macroscopic cartilage assessments were performed following euthanasia at 52 weeks. As found in prior studies, the repair group had significantly less cartilage damage than either the ACL transected or ACL reconstruction groups (P<.03). The presence and concentrations of the biomarkers were influenced by surgical group and time. In general, the concentrations of inflammatory mediators were higher in the repair group, which exhibited less cartilage damage than the other two treatment groups. While this finding disproved the hypotheses, these data suggest that the metabolic activity of the joints exhibiting less cartilage damage remained higher over the 52-week period than those that did not.