A comparison of ARA and DNA data for microbial source tracking based on source-classification models developed using classification trees.
A comparison of ARA and DNA data for microbial source tracking based on source-classification models developed using classification trees.
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基于使用分类树开发的源分类模型，比较用于微生物源追踪的 ARA 和 DNA 数据。
DOI：
10.1016/j.watres.2007.05.026
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发表时间：
2007
期刊：
影响因子：
12.8
通讯作者：
A. Ware
中科院分区：
文献类型：
--
作者：
B. Price;E. Venso;M. Frana;Joshua Greenberg;A. Ware
有关微生物源追踪 (MST) 的文献表明，与抗生素耐药性分析 (ARA) 相比，粪便样本 DNA 分析可以更可靠地确定地表水污染的细菌来源。我们的目标是确定使用 DNA 数据开发的基于库的 MST 的可靠性（如果有的话）的提高是否足以证明其较高的成本是合理的，其中细菌源预测用于 TMDL 地表水管理项目。我们描述了 MST 分类树的应用，该树应用于马里兰州波托马克河流域收集的样本中的 ARA 和 DNA 数据。关于 ARA 和 DNA 数据比较的结论虽然目前还处于初步阶段，但表明在 MST 应用于 TMDL 地表水管理计划的情况下，与 ARA 数据的成本相比，获取 DNA 数据的额外成本可能不合理。
The literature on microbial source tracking (MST) suggests that DNA analysis of fecal samples leads to more reliable determinations of bacterial sources of surface water contamination than antibiotic resistance analysis (ARA). Our goal is to determine whether the increased reliability, if any, in library-based MST developed with DNA data is sufficient to justify its higher cost, where the bacteria source predictions are used in TMDL surface water management programs. We describe an application of classification trees for MST applied to ARA and DNA data from samples collected in the Potomac River Watershed in Maryland. Conclusions concerning the comparison of ARA and DNA data, although preliminary at the current time, suggest that the added cost of obtaining DNA data in comparison to the cost of ARA data may not be justified, where MST is applied in TMDL surface water management programs.