Honey Bee Thermal/Chemical Sensor, AmHsTRPA, Reveals Neofunctionalization and Loss of Transient Receptor Potential Channel Genes
Honey Bee Thermal/Chemical Sensor, AmHsTRPA, Reveals Neofunctionalization and Loss of Transient Receptor Potential Channel Genes
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DOI：
10.1523/jneurosci.2001-10.2010
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发表时间：
2010-09-15
影响因子：
5.3
通讯作者：
Kadowaki, Tatsuhiko
中科院分区：
文献类型：
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作者：
Kohno, Keigo;Sokabe, Takaaki;Kadowaki, Tatsuhiko
昆虫是相对较小的变温动物，因此它们对环境温度的变化非常敏感。然而，一群蜜蜂能够通过冷却或加热巢来将巢的温度保持在32摄氏度至36摄氏度之间。然而，蜜蜂如何感知环境温度还不清楚。我们确定了一个蜜蜂Hyatelera特异性瞬时受体电位A（HsTRPA）通道（AmHsTRPA），这是激活的热与表观阈值温度为34摄氏度和昆虫拒食剂，如樟脑在体外。AmHsTRPA在触角鞭毛中表达，并且触角鞭毛的消融和AmHsTRPA抑制剂的注射损害蜜蜂的避温。蜜蜂对蔗糖的味觉反应被有害热和昆虫拒食剂抑制，但在AmHsTRPA抑制剂的存在下被缓解。这些结果表明，AmHsTRPA可以作为一个热/化学传感器在体内。如前所述，Hyplitera已经失去了古老的化学传感器TRPA 1;然而，AmHsTRPA能够补充果蝇TRPA 1的功能。这些结果表明，HsTRPA，最初由水女巫的复制产生，已经获得了热和化学响应特性，这导致了古老的TRPA 1的丢失。因此，这是复制的离子通道基因的新功能化，随后是功能等同的古老基因的丢失的实例。
Insects are relatively small heterothermic animals, thus they are highly susceptible to changes in ambient temperature. However, a group of honey bees is able to maintain the brood nest temperature between 32 degrees C and 36 degrees C by either cooling or heating the nest. Nevertheless, how honey bees sense the ambient temperature is not known. We identified a honey bee Hymenoptera-specific transient receptor potential A (HsTRPA) channel (AmHsTRPA), which is activated by heat with an apparent threshold temperature of 34 degrees C and insect antifeedants such as camphor in vitro. AmHsTRPA is expressed in the antennal flagellum, and ablation of the antennal flagella and injection of AmHsTRPA inhibitors impair warmth avoidance of honey bees. Gustatory responses of honey bees to sucrose are suppressed by noxious heat and insect antifeedants, but are relieved in the presence of AmHsTRPA inhibitors. These results suggest that AmHsTRPA may function as a thermal/chemical sensor in vivo. As shown previously, Hymenoptera has lost the ancient chemical sensor TRPA1; however, AmHsTRPA is able to complement the function of Drosophila melanogaster TRPA1. These results demonstrate that HsTRPA, originally arisen by the duplication of Water witch, has acquired thermal- and chemical-responsive properties, which has resulted in the loss of ancient TRPA1. Thus, this is an example of neofunctionalization of the duplicated ion channel gene followed by the loss of the functionally equivalent ancient gene.