Effects of Chronic Cadmium Poisoning on Zn, Cu, Fe, Ca, and Metallothionein in Liver and Kidney of Rats
Effects of Chronic Cadmium Poisoning on Zn, Cu, Fe, Ca, and Metallothionein in Liver and Kidney of Rats
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DOI：
10.1007/s12011-012-9394-9
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发表时间：
2012-03
影响因子：
3.9
通讯作者：
Ding Zhang;Jianfeng Gao;Kerong Zhang;Xiaodong Liu;Jia-kui Li
中科院分区：
文献类型：
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作者：
Ding Zhang;Jianfeng Gao;Kerong Zhang;Xiaodong Liu;Jia-kui Li
研究慢性镉中毒对大鼠肝、肾组织锌、铜、铁、钙及金属硫蛋白基因表达和蛋白质合成的影响。将40只6周龄大鼠随机分为两组。A组给予CdCl 2（1 mg/kgCd ~（2+））腹腔注射，1次/d。对照组给予生理盐水灌胃。在第三周结束时收集肝脏和肾脏用于分析。结果表明，镉暴露使肝脏和肾脏中镉和锌含量显著增加（P <0.01）。Fe和Ca在肾脏中含量显著增加（P< 0.01），而在肝脏中含量均不同程度降低。肝、肾组织MT mRNA和蛋白质表达不一致。肝脏MT mRNA和蛋白表达均显著增加（P< 0.01），肾脏MT基因表达增加（P< 0.01）。MT-1和MT-2 mRNA在肝、肾组织中的表达水平存在差异。本研究表明，组织Cd和Zn水平的变化往往反映MT mRNA的表达，但没有明确的关系MT蛋白。镉含量、MT基因表达和MT蛋白合成之间没有严格的剂量依赖关系。Zn、Fe、Cu、Ca的变化与MT mRNA和蛋白的变化有一定的交互作用。
An experiment was conducted to invest effects of chronic cadmium poisoning on Zn, Cu, Fe, Ca, and metallothionein gene expression and protein synthesis in liver and kidney in rats. Forty rats, 6 weeks old, were randomly allocated into two groups. A group was given CdCl2(1 mg/KgCd2+) by intraperitoneal injection once a day. The other group was treated with normal saline in the same way. Liver and kidney were collected for analysis at the end of the third week. Results showed that Cd exposure increased Cd (P< 0.01) and Zn (P< 0.01) content both in liver and kidney. Fe and Ca concentration had a considerable increase in kidney (P< 0.01), while both had different degree reduction in liver. Discrepancies between MT mRNA and protein were observed in liver and kidney. In liver, both MT mRNA and protein had a significant increase (P< 0.01), while in kidney, only MT gene increase was checked. Meanwhile, the expression levels of MT-1 mRNA and MT-2 mRNA were distinct between liver and kidney. The present study indicated that changes in tissue Cd and Zn levels tended to reflect MT mRNA expression, but bear no clear relationship with MT protein. There did not have a strict dose-dependent relationship among Cd content, MT gene expression, and MT protein synthesis. What is more, changes of Zn, Fe, Cu, and Ca had a certain interaction with both MT mRNA and protein.