Construction of refreshable microfluidic channels and electrophoresis along them
Construction of refreshable microfluidic channels and electrophoresis along them
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可刷新微流体通道的构建和沿其电泳
DOI：
10.1016/j.snb.2005.05.015
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Masashi Watanabe
中科院分区：
文献类型：
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作者：
Masashi Watanabe
我们提出并证明了一个“可刷新的微流体通道。”该通道在两个堆叠的载玻片之间构建，具有100μm厚的间隔物。通过用磨料研磨使载玻片的每个表面磨砂，并由于用1，1，1，3，3，3-六甲基二硅氮烷处理而使其疏水。使用二甲基亚砜（DMSO）作为墨水在表面上画线。因为这种DMSO线可以像启动水一样工作，所以水溶液沿着线流动并形成通道，尽管表面上没有真实的凹槽。该通道可以通过用水冲洗载玻片而容易地擦除。将载玻片在空气中干燥后，我们可以在其上重建另一个通道。我们实际获得的通道宽度范围为470至950μm。此外，我们还演示了染料（溴酚蓝）在通道中的电泳。
We proposed and demonstrated a “refreshable microfluidic channel.” This channel was constructed between two stacked glass slides with a 100μm thick spacer. Each surface of the slides was frosted by being ground with an abrasive and made hydrophobic due to the treatment with 1,1,1,3,3,3-hexamethyldisilazane. A line was drawn on the surface using dimethyl sulfoxide (DMSO) as the ink. Because this DMSO line could work like priming water, a water solution flowed along the line and a channel formed, though there was no real groove on the surface. This channel could be easily erased by rinsing the slide with water. After drying the slide in air, we could reconstruct another channel on it. The channel widths that we actually obtained ranged from 470 to 950μm. In addition, we demonstrated the electrophoresis of a dye (bromophenol blue) in the channel.