MALDI-TOF MS analysis of human and animal Brachyspira species and benefits of database extension
MALDI-TOF MS analysis of human and animal Brachyspira species and benefits of database extension
复制标题
DOI：
10.1016/j.jprot.2012.09.027
复制
发表时间：
2013-01-14
影响因子：
3.3
通讯作者：
Chezzi, Carlo
中科院分区：
文献类型：
--
作者：
Calderaro, Adriana;Piccolo, Giovanna;Chezzi, Carlo
属于短螺旋体属的螺旋体属是定殖于人类和动物（主要是猪）的大肠的厌氧细菌。主要种类为B.猪痢疾病原菌猪疫霉B. B.一种人畜共患病病原体。人和不同动物物种的结肠螺旋体病，B。aalborgi，专门感染人类引起结肠螺旋体病，B。中间型，一种潜在的动物病原体，B. innocens和B. murdochii，一般为猪的腹型; B.在这项研究中，首次将MALDI-TOF MS应用于人类和动物来源的短螺旋体菌株，补充了现有的数据库，仅限于物种B。murdochii的螺旋体蛋白质谱，并证明其在快速，廉价和可靠的鉴定短螺旋体菌株在物种水平上的有用性，克服了以前在鉴定这些螺旋体时使用基于生物化学和遗传学的方法时遇到的问题。此外，基于不同螺旋体物种的蛋白质谱生成了反映其宿主谱的树状图，显示在同一分支中仅有两个能够感染人类的物种（B. aalborgi和B.在另一个分支中是专门感染动物的螺旋体。(C)2012爱思唯尔有限公司版权所有。
Spirochaetes belonging to the genus Brachyspira are anaerobic bacteria that colonize the large intestine of humans and animals, mainly pigs. The main species are namely, B. hyodysenteriae, the etiological agent of swine dysentery, B. pilosicoli, a zoonotic agent causing. colonic spirochaetosis both in humans and different animal species, B. aalborgi, exclusively infecting humans causing colonic spirochaetosis, B. intermedia, a potential animal pathogen, B. innocens and B. murdochii, generally commensal of pigs, and B. alvinipulli, found in egg laying hens with diarrhea.In this study, for the first time, MALDI-TOF MS was applied on Brachyspira strains of human and animal origins, supplementing the existing database, limited to the species B. murdochii only, with spirochaetal protein profiles and demonstrating its usefulness in the rapid, cheap and reliable identification of Brachyspira strains at the species level, overcoming the problems previously encountered in the identification of these spirochaetes when using biochemical and genetic-based methods.Moreover, a dendrogram based on protein profiles of the different spirochaetal species was generated reflecting their host spectrum, showing in the same branch the only two species able to infect humans (B. aalborgi and B. pilosicoli) and in the other branch the spirochaetes infecting exclusively animals. (C) 2012 Elsevier B.V. All rights reserved.