GLABROUS1 from Rosa roxburghii Tratt regulates trichome formation by interacting with the GL3/EGL3 protein
GLABROUS1 from Rosa roxburghii Tratt regulates trichome formation by interacting with the GL3/EGL3 protein
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来自刺梨的 GLABROUS1 通过与 GL3/EGL3 蛋白相互作用来调节毛状体的形成
DOI：
10.1016/j.gene.2018.12.071
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发表时间：
2019-04-15
期刊：
影响因子：
3.5
通讯作者：
Yi, Yin
中科院分区：
文献类型：
--
作者：
Huang, Xiaolong;Yan, Huiqing;Yi, Yin
GLABRUS1 是 R2R3 MYB 同源物，对于毛状体发育的启动至关重要。本研究利用RACE技术克隆了刺梨全长cDNA，命名为RrGL1，全长1013 bp，其中开放阅读框长792 bp，编码263个氨基酸。原位杂交与qRT-PCR结果相对应，表明RrGL1转录本主要在叶柄、叶和茎中表达。还评估了刺梨不同果实发育阶段的 RrGL1 表达水平。 RrGL1在幼果中高表达，随着果实成熟表达量降低。 RrGL1 的过表达能够在功能上补充拟南芥 gl1(-/-) 突变体的毛状体形成。通过亚细胞定位分析，RrGL1 位于细胞核中，并在酵母双杂交实验中与拟南芥 GL3/EGL3 发生物理相互作用，这表明 RrGL1 可能通过在毛状体起始过程中形成 MYB-碱性螺旋-环-螺旋复合物来发挥功能。刺梨的刺的形成与拟南芥毛状体的刺相似。这些结果为进一步研究刺的形成和发育的分子机制奠定了基础，以协助和培育无刺果实的基因工程。
GLABROUS1 is an R2R3 MYB homolog that is essential for the initiation of trichome development. In this study, we used RACE to clone a full-length cDNA from Rosa roxburghii Tratt, termed RrGL1, which was 1013 bp, including an open reading frame of 792 bp that encoded 263 amino acids. In situ hybridization, corresponding with qRT-PCR results, revealed that RrGL1 transcripts were mainly expressed in petiole, leaf, and stem. RrGL1 expression levels at various fruit developmental stages in R. roxburghii were also evaluated. RrGL1 was highly expressed in young fruit, and the expression level decreased with fruit maturation. The overexpression of RrGL1 was able to functionally complemented the Arabidopsis thaliana gl1(-/-) mutant in trichome formation. RrGL1 was located in the cell nucleus with analysis of subcellular localization and physically interacted with A. thaliana GL3/EGL3 in the yeast two-hybrid assay, implying that RrGL1 might exert functions by forming a MYB-basic helix-loop-helix complex in trichome initiation. The formation of prickles in R. roxburghii is similar to that of Arabidopsis trichome.These results provided a foundation for further research on the molecular mechanisms underlying the formation and development of prickles that could assist and cultivate in the genetic engineering of prickle-free fruits.