Protection from yellow head virus (YHV) infection in Penaeus vannamei pre-infected with Taura syndrome virus (TSV)
Protection from yellow head virus (YHV) infection in Penaeus vannamei pre-infected with Taura syndrome virus (TSV)
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DOI：
10.3354/dao02448
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发表时间：
2012-04-26
影响因子：
1.4
通讯作者：
Lightner, Donald V.
中科院分区：
文献类型：
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作者：
Fernando Aranguren, Luis;Tang, Kathy F. J.;Lightner, Donald V.
先暴露于陶拉综合征病毒（TSV），再用黄头病毒（YHV）攻毒后，凡纳美对虾获得了对黄头病（YHD）的部分保护。实验感染采用无特定病原体（SPF）对虾，该对虾首先暴露于TSV；在感染后27、37和47 d，每只虾注射1 × 10(4)份YHV病毒（称为TSV-YHV组）。未感染TSV的对虾注射YHV作为阳性对照。比较TSV-YHV组和YHV（阳性对照）组的生存分析，所有时间组的生存率均显著（p < 0.001）。TSV-YHV组的最终生存率（平均55%）高于阳性对照组（0%）（p < 0.05）。采用双工反转录定量PCR对TSV和YHV进行定量。在多足类(3.52 × 10（9)比1.88 × 10（10）拷贝mu g RNA(-1)）和淋巴器官（LO）样本(3.52 × 10（9)比1.88 × 10（10）拷贝mu g RNA(-1)）中，TSV-YHV组的YHV拷贝数低于阳性对照（p < 0.001）。原位杂交分析发现两种病毒在靶组织中的分布存在差异。LO病灶感染TSV，未感染YHV。这项研究表明，在TSV和YHV之间存在一种病毒干扰效应，这可能部分解释了美洲没有YHD的原因，在美洲，P. vannamei经常在存在TSV的农场中饲养。
Pacific white shrimp Penaeus vannamei that were pre-exposed to Taura syndrome virus (TSV) and then challenged with yellow head virus (YHV) acquired partial protection from yellow head disease (YHD). Experimental infections were carried out using specific-pathogen-free (SPF) shrimp which were first exposed per os to TSV; at 27, 37 and 47 d post infection they were then challenged by injection with 1 x 10(4) copies of YHV per shrimp (designated the TSV-YHV group). Shrimp not infected with TSV were injected with YHV as a positive control. Survival analyses comparing the TSV-YHV and YHV (positive control) groups were conducted, and significant survival rates were found for all the time groups (p < 0.001). A higher final survival was found in the TSV-YHV group (mean 55%) than in the positive control (0%) (p < 0.05). Duplex reverse transcription quantitative PCR was used to quantify both TSV and YHV. Lower YHV copy numbers were found in the TSV-YHV group than in the positive control in pleopods (3.52 x 10(9) vs. 1.88 x 10(10) copies mu g RNA(-1)) (p < 0.001) and lymphoid organ (LO) samples (3.52 x 10(9) vs. 1.88 x 10(10) copies mu g RNA(-1)) (p < 0.01). In situ hybridization assays were conducted, and differences in the distribution of the 2 viruses in the target tissues were found. The foci of LO were infected with TSV but were not infected with YHV. This study suggests that a viral interference effect exists between TSV and YHV, which could, in part, explain the absence of YHD in the Americas, where P. vannamei are often raised in farms where TSV is present.