Ectomycorrhizae of Lactarius lignyotus on Norway spruce, characterized by anatomical and molecular tools
Ectomycorrhizae of Lactarius lignyotus on Norway spruce, characterized by anatomical and molecular tools
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挪威云杉上 Lactarius lignyotus 的外生菌根，通过解剖学和分子工具进行表征
DOI：
10.1007/bf00203334
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发表时间：
1995
期刊：
影响因子：
3.9
通讯作者：
B. Javornik
中科院分区：
文献类型：
--
作者：
H. Kraigher;R. Agerer;B. Javornik
对挪威云杉上乳菇的外生菌根进行了形态和解剖特征的综合描述。外生菌根的鉴定是通过跟踪菌丝体到子实体和分子工具，利用聚合酶链式反应(PCR)扩增真菌DNA进行的。新描述的外生菌根与其近缘种黑乳菇的外生菌根相比较。两种真菌及其外生菌根的DNA扩增产物经HInf1酶切后可通过特征片段进行区分。
The ectomycorrhizae ofLactarius lignyotuson Norway spruce are comprehensively described by morphological and anatomical characteristics. Identification of ectomycorrhizae was performed by tracing mycelia to the fruitbodies and also by molecular tools, using polymerase chain reaction (PCR) amplification of the fungal DNA. The newly described ectomycorrhiza is compared to ectomycorrhiza of the relatedLactarius picinus. The amplified DNA products of the two fungi and their ectomycorrhizae could be distinguished by characteristic fragments after digestion withHinf1.