A Large Scale Laboratory Cage Trial of Aedes Densonucleosis Virus (AeDNV)
A Large Scale Laboratory Cage Trial of Aedes Densonucleosis Virus (AeDNV)
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DOI：
10.1603/me09157
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发表时间：
2010-05-01
影响因子：
2.1
通讯作者：
Black, William C.
中科院分区：
文献类型：
--
作者：
de Valdez, Megan R. Wise;Suchman, Erica L.;Black, William C.
埃及伊蚊（L.）（双翅目：库蚊科）是登革热病毒（DENVI-4）的主要媒介，在人类住所内和周围产卵，包括难以定位的地点，这给控制这种蚊子带来了挑战。探讨了伊蚊登核病病毒（AeDNV）作为伊蚊生物防治剂的有效性和可持续性。埃及伊蚊在大型实验室笼内产卵点和产卵点之间（bb10 - 92米(3)），作为现场试验的前导。在一个单独的产卵点（OPS）和不同距离的未播种的产卵点（OPS）中，选择一个笼子播种AeDNV。采用实时定量聚合酶链反应技术跟踪AeDNV在ops中的扩散和积累情况。每周从每个笼中收集所有鸡蛋并计数。我们的问题是：(1)AeDNV是否分散在不同的距离上，它能否在新的ops中积累和持续存在？(2)在AeDNV处理的种群中，卵密度是否降低？AeDNV在新型ops中分散并持续存在。病毒在ops中的积累与卵密度呈正相关，与初始感染源的接近程度影响病毒扩散的时间和滴度的维持。AeDNV未显著降低Ae。埃及伊蚊卵密度。目前的研究表明，成年雌性伊蚊。埃及伊蚊产卵行为导致病毒成功地从处理过的容器传播到大型笼中的新型容器；然而，达到的AeDNV滴度不足以降低卵密度。
Aedes aegypti (L.) (Diptera: Culicidae) the primary vector of dengue viruses (DENVI-4), oviposit in and around human dwellings, including sites difficult to locate, making control of this mosquito challenging. We explored the efficacy and sustainability of Aedes Densonucleosis Virus (AeDNV) as a biocontrol agent for Ae. aegypti in and among oviposition sites in large laboratory cages (>92 m(3)) as a prelude to field trials. Select cages were seeded with AeDNV in a single oviposition site (OPS) with unseeded OPSs established at varied distances. Quantitative real-time polymerase chain reaction was used to track dispersal and accumulation of AeDNV among OPSs. All eggs were collected weekly from each cage and counted. We asked: (1) Is AeDNV dispersed over varying distances and can it accumulate and persist in novel OPSs? (2) Are egg densities reduced in AeDNV treated populations? AeDNV was dispersed to and sustained in novel OPSs. Virus accumulation in OPSs was positively correlated with egg densities and proximity to the initial infection source affected the timing of dispersal and maintenance of viral titers. AeDNV did not significantly reduce Ae. aegypti egg densities. The current study documents that adult female Ae. aegypti oviposition behavior leads to successful viral dispersal from treated to novel containers in large-scale cages; however, the AeDNV titers reached were not sufficient to reduce egg densities.