A hepatitis E virus variant from the United States: molecular characterization and transmission in cynomolgus macaques
A hepatitis E virus variant from the United States: molecular characterization and transmission in cynomolgus macaques
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DOI：
10.1099/0022-1317-80-3-681
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发表时间：
1999-03-01
影响因子：
3.8
通讯作者：
Mushahwar, IK
中科院分区：
文献类型：
--
作者：
Erker, JC;Desai, SM;Mushahwar, IK
据报道，从美国 (US) 一名患有急性病毒性肝炎且尚无已知感染 HEV 危险因素的患者体内分离出戊型肝炎病毒 (HEV-US1) 的部分序列。这些序列与之前表征的 HEV 分离株显着不同，暗示存在独特的人类变异。在本文中，我们报告了 HEV-US1 和第二个美国分离株 (HEV-US2) 的近全长序列。 HEV-US2 在一名患有急性病毒性肝炎的美国患者中被发现。这些序列证实了北美存在一种新的 HEV 毒株，并提供了有关变异体之间变异程度的信息。 HEV-US 核苷酸序列彼此有 92% 相同，但与缅甸和墨西哥毒株只有 74% 相同。氨基酸和系统发育分析还表明，美国分离株在遗传上是不同的，表明存在三种 HEV 基因型，第二名美国患者的血清在接种食蟹猴后诱发肝炎。2-4 周内，在血清和粪便中均可检测到 HEV-US2 RNA，这与血清丙氨酸转氨酶水平升高一致。接种后 8 周，随着抗体滴度增加，感染得到缓解。
The partial sequence of a hepatitis E virus (HEV-US1) isolated from a patient in the United States (US), suffering from acute viral hepatitis with no known risk factors for acquiring HEV, has been reported. These sequences were significantly different from previously characterized HEV isolates, alluding to the existence of a distinct human variant, In this paper, we report the near full-length sequences of HEV-US1 and a second US isolate (HEV-US2). HEV-US2 was identified in a US patient suffering from acute viral hepatitis. These sequences verify the presence of a new HEV strain in North America and provide information as to the degree of variability between variants. The HEV-US nucleotide sequences are 92% identical to each other and only 74% identical to the Burmese and Mexican strains. Amino acid and phylogenetic analyses also demonstrate that the US isolates are genetically distinct, suggesting the presence of three genotypes of HEV, Serum from the second US patient induced hepatitis following inoculation into a cynomolgus macaque, Within 2-4 weeks, HEV-US2 RNA was detectable in both the serum and faecal material coinciding with elevated serum alanine transaminase levels. Infection resolved as antibody titres increased 8 weeks post-inoculation.