Functional conservation of EXA1 among diverse plant species for the infection by a family of plant viruses
Functional conservation of EXA1 among diverse plant species for the infection by a family of plant viruses
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DOI：
10.1038/s41598-019-42400-w
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发表时间：
2019-04-11
影响因子：
4.6
通讯作者：
Namba, Shigetou
中科院分区：
文献类型：
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作者：
Yusa, Akira;Neriya, Yutaro;Namba, Shigetou
由于植物病毒的繁殖依赖于各种宿主易感因子，因此它们的缺乏可以防止栽培植物和模式植物中的病毒感染。最近，我们在拟南芥中鉴定了马铃薯X病毒积累必需的易感因子1（EXA1），并揭示了EXA1介导的抗性对三种马铃薯X病毒有效。虽然EXA1同源基因在番茄和水稻中被发现，但关于哪些病毒依赖EXA1的感染能力以及EXA1同源基因在病毒感染中的功能是否在包括作物在内的多种植物物种中保守，人们知之甚少。为了解决这些问题，我们在两个茄科物种，本塞姆氏烟草和番茄中使用病毒诱导的基因沉默产生敲除突变体。在N.在本塞姆氏番茄中，EXA1同源物的沉默显著损害了马铃薯X病毒和马铃薯X病毒的近亲Iolavirus的积累，而来自番茄和水稻的EXA1同源物的瞬时表达补充了病毒感染。马铃薯X病毒感染的EXA1依赖性在番茄中也是保守的。这些结果表明，EXA1是马铃薯X病毒和一种Iolavirus的有效积累所必需的，并且EXA1在病毒感染中的功能在不同的植物物种中是保守的。
Since the propagation of plant viruses depends on various host susceptibility factors, deficiency in them can prevent viral infection in cultivated and model plants. Recently, we identified the susceptibility factor Essential for poteXvirus Accumulation 1 (EXA1) in Arabidopsis thaliana, and revealed that EXA1-mediated resistance was effective against three potexviruses. Although EXA1 homolog genes are found in tomato and rice, little is known about which viruses depend on EXA1 for their infection capability and whether the function of EXA1 homologs in viral infection is conserved across multiple plant species, including crops. To address these questions, we generated knockdown mutants using virus-induced gene silencing in two Solanaceae species, Nicotiana benthamiana and tomato. In N. benthamiana, silencing of an EXA1 homolog significantly compromised the accumulation of potexviruses and a lolavirus, a close relative of potexviruses, whereas transient expression of EXA1 homologs from tomato and rice complemented viral infection. EXA1 dependency for potexviral infection was also conserved in tomato. These results indicate that EXA1 is necessary for effective accumulation of potexviruses and a lolavirus, and that the function of EXA1 in viral infection is conserved among diverse plant species.