Lyophilized Kit for the Preparation of the PET Perfusion Agent [(68)Ga]-MAA.
Lyophilized Kit for the Preparation of the PET Perfusion Agent [(68)Ga]-MAA.
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DOI：
10.1155/2014/269365
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
McGoron, Anthony J
中科院分区：
文献类型：
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作者：
Amor-Coarasa, Alejandro;Milera, Andrew;McGoron, Anthony J
医学成像领域的快速发展为使用正电子发射标记微粒开辟了新的途径。我们研究中使用的放射性同位素是（68）Ga，它很容易从发生器中获得，并且具有良好的PET成像核性质。方法。将市售的大聚集白蛋白（MAA）微粒悬浮在无菌生理盐水中，离心去除游离白蛋白和氯化亚锡，再干燥，保存以供稍后用（68）Ga标记。在不同温度和时间下进行标记。（68）对Ga净化设置也进行了测试和优化。将再干燥MAA微粒与未再干燥MAA微粒的标记率和纯度进行比较。结果。再干燥后MAA颗粒保持原有的尺寸分布。当使用（68）Ga纯化系统时，标记收率在75°C下为98%，而未纯化的（68）Ga则为80%。放射化学纯度在标记后4小时仍超过97%。再干燥MAA和标记方法消除了对最终产品的离心纯化的需要，简化了标记过程。动物实验表明，PET制剂具有较高的体内稳定性，4小时后仍有95%以上的活性留在肺部。
Rapid developments in the field of medical imaging have opened new avenues for the use of positron emitting labeled microparticles. The radioisotope used in our research was (68)Ga, which is easy to obtain from a generator and has good nuclear properties for PET imaging. Methods. Commercially available macroaggregated albumin (MAA) microparticles were suspended in sterile saline, centrifuged to remove the free albumin and stannous chloride, relyophilized, and stored for later labeling with (68)Ga. Labeling was performed at different temperatures and times. (68)Ga purification settings were also tested and optimized. Labeling yield and purity of relyophilized MAA microparticles were compared with those that were not relyophilized. Results. MAA particles kept their original size distribution after relyophilization. Labeling yield was 98% at 75°C when a (68)Ga purification system was used, compared to 80% with unpurified (68)Ga. Radiochemical purity was over 97% up to 4 hours after the labeling. The relyophilized MAA and labeling method eliminate the need for centrifugation purification of the final product and simplify the labeling process. Animal experiments demonstrated the high in vivo stability of the obtained PET agent with more than 95% of the activity remaining in the lungs after 4 hours.