Murine susceptibility to Chagas' disease maps to chromosomes 5 and 17
Murine susceptibility to Chagas' disease maps to chromosomes 5 and 17
复制标题
DOI：
10.1038/sj.gene.6363972
复制
发表时间：
2003-07-01
影响因子：
5
通讯作者：
Fleischer, B
中科院分区：
文献类型：
--
作者：
Graefe, SEB;Meyer, BS;Fleischer, B
恰加斯病是由原生动物寄生虫克氏锥虫引起的，通常在近交系小鼠中建模。小鼠品系对实验感染的敏感性差异很大。我们通过真实的时间PCR定量了抗性和敏感品系中的寄生虫组织负荷，并应用回交策略来绘制与近交系小鼠易感性相关的基因组位点。将耐药的B6D2F1小鼠与易感的C57BL 16小鼠回交，总共192只后代中有46只在感染后死亡。他们的基因组被微卫星标记扫描。17号染色体上的一个区域与易感性显著相关，而5号染色体上的另一个区域暗示了连锁。
Chagas' disease is caused by the protozoan parasite Trypanosoma cruzi and commonly modelled in inbred mice. Susceptibility of mouse strains to experimental infection varies considerably. We quantified parasite tissue burdens in resistant and susceptible strains by real time PCR and applied a backcross strategy to map the genomic loci linked to susceptibility in inbred mice. Resistant B6D2F1 mice were backcrossed with susceptible C57BL16 mice, and 46 of a total 192 offspring died after infection. Their genomes were scanned with microsatellite markers. One region on chromosome 17 was significantly linked to susceptibility, while another on chromosome 5 was suggestive of linkage.