1700108J01Rik and 1700101O22Rik are mouse testis-specific long non-coding RNAs
1700108J01Rik and 1700101O22Rik are mouse testis-specific long non-coding RNAs
复制标题
DOI：
10.1007/s00418-018-1642-4
复制
发表时间：
2018-02
影响因子：
2.3
通讯作者：
Xiaohui Song;Chaw Kyi-Tha-Thu;T. Takizawa;B. T. Naing;Toshihiro Takizawa
中科院分区：
文献类型：
--
作者：
Xiaohui Song;Chaw Kyi-Tha-Thu;T. Takizawa;B. T. Naing;Toshihiro Takizawa
长的非编码RNA（lncRNA;长度> 200个核苷酸）作为基因表达的微调因子已经引起了人们的注意。然而，很少有人知道的细胞和阶段特异性表达模式和功能的lncRNA在精子发生。本研究的目的是使用计算和实验方法的组合来识别小鼠睾丸相关的lncRNA。我们首先使用FANTOM 5数据库调查lncRNA在小鼠睾丸中的表达，并进行逆转录定量聚合酶链反应（实时PCR）和原位杂交（ISH）分析。计算机模拟分析表明，大多数在成年小鼠睾丸中高表达的lncRNA是睾丸特异性lncRNA，并且在精子发生开始时和之后表达。我们从成人睾丸中高表达的lncRNA中选择反义lncRNA 1700108 J 01 Rik和长基因间非编码RNA 1700101 O22 Rik进行进一步分析。Real-time PCR分析证实1700108 J 01 Rik和1700101 O22 Rik在睾丸中特异表达。ISH分析表明，这两种小鼠睾丸特异性lncRNA仅在减数分裂前期和圆形精子细胞阶段的睾丸生殖细胞中表达，这与精子发生过程中转录再激活的时期相吻合。这些lncRNA的细胞质分布揭示ISH表明他们参与转录后基因调控，而不是在表观遗传或转录调控。我们的数据提供了新的见解，睾丸相关的lncRNA，将是有用的表达和功能的研究精子发生。
Long non-coding RNAs (lncRNAs; > 200 nucleotides in length) have attracted attention as fine-tuners of gene expression. However, little is known about the cell- and stage-specific expression pattern and function of lncRNAs in spermatogenesis. The purpose of this study was to identify mouse testis-associated lncRNAs using a combination of computational and experimental approaches. We first used the FANTOM5 database to survey lncRNA expression in the mouse testis and performed reverse transcription quantitative polymerase chain reaction (real-time PCR) and in situ hybridization (ISH) analyses. In silico analysis showed that most of the highly expressed lncRNAs in the adult mouse testis were testis-specific lncRNAs and were expressed at and following the initiation of spermatogenesis. We selected the antisense lncRNA1700108J01Rikand long intergenic non-coding RNA1700101O22Rikfrom the most highly expressed lncRNAs in the adult testis for further analysis. Real-time PCR analysis confirmed that1700108J01Rikand1700101O22Rikwere specifically expressed in the testis. ISH analysis revealed that the two mouse-testis-specific lncRNAs were expressed exclusively in testicular germ cells in meiotic prophase and the round spermatid stage, which coincide with the period of transcriptional reactivation during spermatogenesis. The cytoplasmic distribution of these lncRNAs revealed by ISH suggests their involvement in post-transcriptional gene regulation rather than in epigenetic or transcriptional regulation. Our data provide new insight into testis-associated lncRNAs that will be useful in expression and functional studies of spermatogenesis.