REGENERATING AXONS ENHANCE DIFFERENTIATION OF PERINEURIAL-LIKE CELLS INVOLVED IN MINIFASCICLE FORMATION IN THE INJURED PERIPHERAL-NERVE
REGENERATING AXONS ENHANCE DIFFERENTIATION OF PERINEURIAL-LIKE CELLS INVOLVED IN MINIFASCICLE FORMATION IN THE INJURED PERIPHERAL-NERVE
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DOI：
10.1097/00005072-199411000-00006
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发表时间：
1994-11-01
影响因子：
3.2
通讯作者：
SKETELJ, J
中科院分区：
文献类型：
--
作者：
POPOVIC, M;BRESJANAC, M;SKETELJ, J
研究了周围神经损伤后再生轴突在神经内膜重建成小束中的作用。研究了挤压大鼠坐骨神经的控制远端残端和远端神经残端中，除了其他细胞元素，原来的神经束膜也被破坏的冷冻和解冻。检查其中轴突再生已被阻止的受损神经以进行比较。光镜及超微结构观察其变化。神经损伤后两周，在所有动物组的远端神经残端中观察到通过增殖神经内膜成纤维细胞样细胞进行的初期神经内膜重建。到4周时，对照再生神经（仅挤压）中的神经内膜变化未显示出进展。相反，在原始神经束膜屏障已被消除的远端神经残端中观察到广泛的神经内膜小束重组。在有再生轴突的神经中，包围小束的细胞看起来像分化的神经束膜细胞，而在没有再生轴突的情况下，包围神经膜管的神经内膜细胞大多缺乏神经束膜细胞的典型特征。因此，持续的神经束膜损伤是诱导完全的神经内膜区室化所必需的。在这种情况下，再生轴突促进神经内膜成纤维细胞样细胞分化为神经束膜细胞。
The role of regenerating axons in the restructuring of the endoneurium into minifascicles after peripheral nerve injury was investigated. Endoneurial changes were studied in the control distal stumps of crushed rat sciatic nerves and in the distal nerve stumps in which, in addition to other cellular elements, the original perineurium had also been destroyed by freezing and thawing. Injured nerves in which axonal regeneration had been prevented were examined for comparison. The changes were observed at light microscopic and ultrastructural levels. Two weeks after nerve injury, incipient endoneurial restructuring by proliferating endoneurial fibroblast-like cells was observed in the distal nerve stumps in all groups of animals. By 4 weeks, the endoneurial changes in control regenerating nerves (crush only) displayed no progress. On the contrary, extensive minifascicular reorganization of the endoneurium was observed in the distal nerve stumps in which the original perineurial barrier had been eliminated. The cells that encircled the minifascicles in the nerves with regenerating axons looked like differentiated perineurial cells, whereas in the absence of regenerating axons, the endoneurial cells that encircled the neurilemmal tubes mostly lacked typical features of the perineurial cells. Therefore, persistent perineurial damage is necessary to induce complete endoneurial compartmentalization. In this case, the regenerating axons enhance differentiation of the endoneurial fibroblast-like cells into the perineurial cells.