Inhibition of 125I-human follicle-stimulating hormone binding to receptor by a low molecular weight fraction of bovine follicular fluid: inhibitor concentration is related to biochemical parameters of follicular development.
Inhibition of 125I-human follicle-stimulating hormone binding to receptor by a low molecular weight fraction of bovine follicular fluid: inhibitor concentration is related to biochemical parameters of follicular development.
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牛卵泡液的低分子量部分抑制 125I-人卵泡刺激激素与受体的结合：抑制剂浓度与卵泡发育的生化参数有关。
DOI：
10.1095/biolreprod29.5.1105
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发表时间：
1983
影响因子：
3.6
通讯作者：
L. Reichert
中科院分区：
文献类型：
--
作者：
P. Sluss;P. Fletcher;L. Reichert
卵泡液（FF）池取自怀孕或动情周期黄体期奶牛的大卵泡或小卵泡。通过离心收集每个 FF 池中的细胞，并测量卵泡刺激素 (FSH) 和黄体生成素 (LH) 受体的含量。通过放射免疫分析（RIA）对 FF 中的类固醇水平进行定量。由于牛滤泡细胞（主要是颗粒细胞）的数量有限，因此利用小牛睾丸受体系统研究了 FSH 结合抑制。通过透析制备的低（小于 6000）分子量 (Mr) 级分显示出在未分级 FF 中存在的 FSH 结合抑制 (FSH-BI) 的大部分（76% 至 94%）。发情周期黄体期奶牛的 FF 池中低 Mr FSH-BI 的浓度高于怀孕奶牛的 FF 池中的浓度。从怀孕或黄体期奶牛的小卵泡汇集的 FF 中低 Mr FSH-BI 的浓度也高于从大卵泡汇集的 FF 中。颗粒细胞上促性腺激素（FSH、LH）受体的相对浓度用于根据卵泡成熟的相对程度对池进行排序。卵泡成熟的其他参数是 FF 中雌激素的浓度和雌激素与雄激素的比率。卵泡闭锁的生化参数是 FF 中雄激素的浓度和雌激素与雄激素的比率。（摘要截断为 250 字）
Pools of follicular fluid (FF) were obtained from large or small follicles of cows which were pregnant or in the luteal phase of the estrous cycle. Cells present in each FF pool were collected by centrifugation and measured for content of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) receptors. Steroid levels in FF were quantitated by radioimmunoassay (RIA). Since the quantity of bovine follicular cells (mostly granulosa cells) was limited, FSH binding inhibition was studied utilizing a calf testis receptor system. Low (less than 6000) molecular weight (Mr) fractions prepared by dialysis were shown to account for most (76 to 94%) of the FSH binding inhibition (FSH-BI) present in unfractionated FF. The concentration of low Mr FSH-BI was higher in pools of FF from cows in the luteal phase of the estrous cycle than in pools of FF from pregnant cows. The concentration of low Mr FSH-BI was also higher in FF pooled from small follicles than in FF pooled from large follicles of either pregnant or luteal phase cows. Relative concentrations of receptors for gonadotropins (FSH, LH) on granulosa cells were used to rank the pools according to relative degree of follicular maturation. Other parameters of follicular maturation were concentration of estrogens and the ratio of estrogens to androgens in FF. Biochemical parameters for follicular atresia were the concentration of androgens and the ratio of estrogens to androgens in FF.(ABSTRACT TRUNCATED AT 250 WORDS)