Selection of serotype-specific vaccine candidate genes in Actinobacillus pleuropneumoniae and heterologous immunization with Propionibacterium acnes.
Selection of serotype-specific vaccine candidate genes in Actinobacillus pleuropneumoniae and heterologous immunization with Propionibacterium acnes.
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DOI：
10.1016/j.vaccine.2008.09.039
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发表时间：
2008-11
期刊：
影响因子：
5.5
通讯作者：
L. Lei;Changjiang Sun;S. Lu;Xin Feng;Jiaqi Wang;W. Han
中科院分区：
文献类型：
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作者：
L. Lei;Changjiang Sun;S. Lu;Xin Feng;Jiaqi Wang;W. Han
胸膜肺炎放线杆菌（Actinobacillus pleuropneumoniae，A.胸膜肺炎）是猪胸膜肺炎的高度传染性致死病原体。这种疾病的疫苗通常是血清型特异性的。为了筛选血清型特异性免疫原性基因，我们构建了两个A.利用cDNA代表性差异分析（cDNA-RDA）技术构建了猪胸膜肺炎CCVC 259（血清1型）和CCVC 263（血清5型）的cDNA克隆。从文库中，6个潜在的疫苗候选基因仅在血清1型和血清5型中表达的13个基因，通过抗体筛选后，在体外基因表达与核糖体展示系统。其中8个序列与痤疮丙酸杆菌中的相应基因具有77-100%的同一性。制备了抗A.胸膜肺炎血清型1和5与痤疮丙酸杆菌反应，滴度为1：6400，反之亦然（ELISA滴度，1：3200）。用痤疮丙酸杆菌免疫小鼠，可抵抗10× LD_（50）A.胸膜肺炎血清型1和5，存活率分别为90%和95%。用该菌株免疫猪，可产生高水平的与A.胸膜肺炎，并获得明显的保护，从A。胸膜肺炎感染这些结果表明，A.这种交叉保护性突出了使用痤疮丙酸杆菌疫苗预防胸膜肺炎杆菌和痤疮丙酸杆菌感染的可能性。胸膜肺炎
Actinobacillus pleuropneumoniae (A. pleuropneumoniae) is a highly contagious lethal causative agent of swine pleuropneumoniae. Vaccines for this disease are usually serotype specific. In order to identify immunogenic genes specific to serotypes, two differentially expressed gene cDNA libraries of A. pleuropneumoniae CCVC259 (serotype 1) and CCVC263 (serotype 5) had been constructed by using a cDNA representational difference analysis (cDNA-RDA). From the libraries, six potential vaccine candidate genes expressed only in serotype 1 and 13 genes in serotype 5 were identified by antibody screening after gene expression in vitro with a ribosome display system. Eight sequences out of these exhibited 77–100% identity to the corresponding genes in Propionibacterium acnes. The antisera raised against A. pleuropneumoniae serotypes 1 and 5 were reactive with P. acnes at a titer of 1:6400 and vice versa (ELISA titer, 1:3200). Mice immunized with P. acnes were protected against 10×LD50challenge with A. pleuropneumoniae serotypes 1 and 5, and the survival rates were 90% and 95%, respectively. Pigs vaccinated with the P. acnes strain could develop high level antibody cross-reacted with A. pleuropneumoniae and obtain noticeable protection from A. pleuropneumoniae infection. These data demonstrate that there were common antigens between A. pleuropneumoniae and P. acnes, and the cross protectivity highlights the possibility of using P. acnes vaccines for preventing infection by A. pleuropneumoniae.