Molecular expression of gastrokine 1 in normal mucosa and in Helicobacter pylori-related preneoplastic and neoplastic gastric lesions
Molecular expression of gastrokine 1 in normal mucosa and in Helicobacter pylori-related preneoplastic and neoplastic gastric lesions
复制标题
DOI：
10.4161/cbt.7.12.6936
复制
发表时间：
2008-12-01
影响因子：
3.6
通讯作者：
Arcari, Paolo
中科院分区：
文献类型：
--
作者：
Nardone, Gerardo;Martin, Giovanni;Arcari, Paolo
我们分析了胃粘膜中最丰富的蛋白之一Gastrokine 1（GKN 1）在幽门螺杆菌相关的胃癌前病变和胃癌病变中的表达。pylori阳性者29例。幽门螺杆菌阴性的受试者，通过Western blot，逆转录聚合酶链反应（RT-PCR）和免疫组化进行评估，显示从慢性胃炎到萎缩和肠上皮化生的进行性下降。有趣的是，在胃癌（GC）患者中，GKN 1在组织肿瘤区域中检测不到，但在相应的组织非肿瘤区域中表达。结论GKN 1的表达与胃粘膜炎症损伤有关，并可能与胃细胞表型有关。
We have analyzed the expression of Gastrokine 1 (GKN1), one of the most abundant protein of gastric mucosa, in Helicobacter pylori-related preneoplastic and neoplastic gastric lesions.The GKN1 expression was downregulated in 36 H. pylori-positive patients with respect to 29 H. pylori-negative subjects as evaluated by Western blot, reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry, showing a progressive decrease from chronic gastritis to atrophy and intestinal metaplasia. Interestingly, in gastric cancer (GC) patients, GKN1 was undetectable in tissue tumoral areas but was instead expressed in the corresponding tissue non-tumoral areas. In conclusion our data suggests that GKN1 expression is related to the inflammatory damage of gastric mucosa and could be the related to the gastric cellular phenotype.