Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies.
Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies.
复制标题
测量跨内皮电阻 (TEER) 以进行登革热感染研究。
DOI：
10.1007/978-1-0716-1879-0_13
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Mackow,ErichR
中科院分区：
文献类型：
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作者：
Conde,JonasNascimento;Mladinich,Megan;Schutt,William;Mackow,ErichR
越来越多的证据表明，内皮细胞（EC）在登革病毒（DENV）感染中观察到的免疫增强病理学中起着重要作用，这可能导致严重登革病例中的血管通透性和出血表现。然而，仍然存在一个问题，即DENV感染EC是否直接导致渗透性，或者是否需要诸如免疫细胞活化或抗体依赖性增强（ADE）等内皮外因素。在这一章中，我们详细介绍了跨内皮电阻（TEER）的测量，这是一种定量技术，用于测量内皮细胞单层细胞培养模型中紧密连接动力学的完整性，并表明内皮细胞的DENV感染在体外不会引起内皮渗透性。
A growing body of evidence demonstrates that endothelial cells (ECs) play a prominent role in immune-enhanced pathology seen in dengue virus (DENV) infection that might contribute to vascular permeability and hemorrhagic manifestations in severe dengue cases. However, it remains a question of whether DENV infection of ECs directly causes permeability or if extra-endothelial factors such as immune cell activation or antibody-dependent enhancement (ADE) are required. In this chapter, we detail the measurement of the transendothelial electrical resistance (TEER), a quantitative technique to measure the integrity of tight junction dynamics in cell culture models of endothelial monolayers and show that DENV infection of ECs does not cause endothelial permeability in vitro.