HPV infection and p53 inactivation in pterygium
HPV infection and p53 inactivation in pterygium
复制标题
翼状胬肉中的 HPV 感染和 p53 失活
作者：
Y. Tsai;Chi;Chun;K. Yeh;Pei;Chi;M. Chou;Hue Lee;Ya‐Wen Cheng
目的研究翼状胬肉组织中抑癌基因p53的突变及蛋白质的异常表达。人乳头瘤病毒（HPV）16/18 E6对p53的失活在宫颈肿瘤发生中起着至关重要的作用。在这项研究中，我们进一步推测，p53失活可能与HPV感染在翼状胬肉的发病机制。为了研究HPV 16/18 E6在翼状胬肉p53失活中的作用，本研究分析了HPV 16或HPV 18感染、HPV E6癌蛋白和p53蛋白表达之间的关系。方法采用巢式聚合酶链反应（nested-PCR）检测129例正常结膜组织和20例正常结膜组织中HPV 16/18感染，直接测序法检测p53突变，免疫组化法检测p53和HPV 16/18 E6蛋白。结果HPV 16/18在翼状胬肉组织中的阳性率为24%（31/129），而在正常结膜组织中均未检测到HPV 16/18 E6蛋白; HPV 16/18阳性的翼状胬肉组织中，HPV 16/18 E6蛋白阳性率为48.3%（15/31）。此外，翼状胬肉中p53蛋白阴性表达与HPV 16/18 E6癌蛋白表达相关，但与p53突变无关。结论HPV 16/18 E6参与了HPV介导的翼状胬肉的发病过程，其部分参与了p53的失活，并在HPVDNA阳性的翼状胬肉中表达。
Purpose Our recent report indicated that tumor suppressor gene (p53) mutations and protein aberrant expression were detected in pterygium. Inactivation of p53 by Human papillomavirus (HPV) 16/18 E6 plays a crucial role in cervical tumorigenesis. In this study, we further speculate that p53 inactivation may be linked with HPV infection in pterygium pathogenesis. To investigate the involvement of HPV 16/18 E6 in p53 inactivation in pterygium, the association between HPV 16 or HPV 18 infection, the HPV E6 oncoprotein, and p53 protein expression was analyzed in this study. Methods HPV 16/18 infection was detected by nested-polymerase chain reaction (nested-PCR), the p53 mutation was detected by direct sequencing, and the p53 and the HPV 16/18 E6 proteins were studied using immunohistochemistry on 129 pterygial specimens and 20 normal conjunctivas. Results The HPV 16/18 was detected in 24% of the pterygium tissues (31 of 129) but not in the normal conjunctiva, and the HPV16/18 E6 oncoprotein was detected in 48.3% of HPV 16/18 DNA-positive pterygium tissues (15 of 31). In addition, p53 protein negative expression in pterygium was correlated with HPV16/18 E6 oncoprotein expression but not with a p53 mutation. Conclusions HPV 16/18 E6 contributes to HPV-mediated pterygium pathogenesis as it is partly involved in p53 inactivation and is expressed in HPV DNA-positive pterygium.
DOI：
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发表时间：
1998
期刊：
The American journal of pathology
影响因子：
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作者：
S. Wilczynski;B. T. Lin;Yuan Xie;Benjamin Pazt
通讯作者：
S. Wilczynski;B. T. Lin;Yuan Xie;Benjamin Pazt
影响因子：
10.5
作者：
WU, XW;BAYLE, JH;LEVINE, AJ
通讯作者：
LEVINE, AJ