Small molecule phosphorescent probes for O2 imaging in 3D tissue models
Small molecule phosphorescent probes for O2 imaging in 3D tissue models
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DOI：
10.1039/c3bm60272a
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发表时间：
2014-01-01
影响因子：
6.6
通讯作者：
Papkovsky, Dmitri B.
中科院分区：
文献类型：
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作者：
Dmitriev, Ruslan I.;Kondrashina, Alina V.;Papkovsky, Dmitri B.
氧合监测对于研究3D组织模型中单个细胞的生长、分化和功能的生理实验非常重要。基于磷光的O-2传感和成像可能允许该任务;然而，当前的探针不提供期望的生物分布和分析性能。我们提出了几种新的细胞穿透磷光共轭物的Pt（II）-四（五氟苯基）卟啉（PtPFPP）染料点击修饰与硫醇产生的，并执行其评价为O-2成像探针的三维组织模型。亲水性葡萄糖（Pt-Glc）和半乳糖（Pt-Gal）缀合物在水性介质中表现出最小的聚集和自猝灭，并且在工作浓度下对不同细胞类型和多细胞聚集体进行有效的深度染色
Monitoring of oxygenation is important for physiological experiments investigating the growth, differentiation and function of individual cells in 3D tissue models. Phosphorescence based O-2 sensing and imaging potentially allow this task; however, current probes do not provide the desired bio-distribution and analytical performance. We present several new cell-penetrating phosphorescent conjugates of a Pt(II)-tetrakis(pentafluorophenyl) porphine (PtPFPP) dye produced by click-modification with thiols, and perform their evaluation as O-2 imaging probes for 3D tissue models. The hydrophilic glucose (Pt-Glc) and galactose (Pt-Gal) conjugates demonstrated minimal aggregation and self-quenching in aqueous media, and efficient in-depth staining of different cell types and multi-cellular aggregates at working concentrations