STIMULATION OF [H-3] THYMIDINE UPTAKE IN MOUSE MARROW BY GRANULOCYTEMACROPHAGE COLONY STIMULATING FACTOR FROM MOUSE LUNG CONDITIONED MEDIUM
STIMULATION OF [H-3] THYMIDINE UPTAKE IN MOUSE MARROW BY GRANULOCYTEMACROPHAGE COLONY STIMULATING FACTOR FROM MOUSE LUNG CONDITIONED MEDIUM
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DOI：
10.1016/0022-1759(83)90417-9
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发表时间：
1983-01-01
影响因子：
2.2
通讯作者：
YAU, OW
中科院分区：
文献类型：
--
作者：
HORAK, H;TURNER, AR;YAU, OW
小鼠肺条件培养基中的粒细胞-巨噬细胞集落刺激因子（粒细胞-巨噬细胞集落刺激因子）造血因子对小鼠骨髓细胞[3H]胸苷摄取的刺激作用，用于跟踪该因子在培养基中的活性，以及其从培养基中部分纯化的过程。该试验在微滴板上进行，比传统的菌落计数更容易和更快。将骨髓细胞置于有该因子存在的平底板中孵育5天，然后用[3H]胸腺嘧啶（2 .mu）标记。[3H]胸腺嘧啶摄取的刺激与半固体甲基纤维素培养中粒细胞-巨噬细胞菌落的发育进行了比较。在（NH4）2 SO4沉淀、Sephaedex凝胶过滤、魔豆蛋白- a - sepharose层析和聚丙烯酰胺凝胶电泳等条件下，两种方法的活性均相同。
Stimulation of [3H]thymidine uptake in mouse marrow cells by a hematopoietic factor, granulocyte-macrophage colony stimulating factor from mouse lung conditioned medium, was used to follow the activity of the factor in the medium, and during its partial purification from the medium. The assay was performed in microtiter plates and was much easier and faster than conventional colony counting. The marrow cells were incubated in flat-bottomed plates in the presence of the factor for 5 days before labeling with [3H]thymidine (2 .mu.Ci/well, 5 Ci/well, 5 Ci/mmol) for 6 h. Stimulation of the [3H]thymidine uptake was compared with the development of granulocyte-macrophage colonies in semisolid methylcellulose culture. The activity followed by both assays showed identical behavior when subjected to (NH4)2 SO4 precipitation, Sephaedex gel filtration, concanavalin-A-Sepharose chromatography and polyacrylamide gel electrophoresis.