Induced alkoxyresorufin-O-dealkylases in tilapias (Oreochromis niloticus) from Guandu river, Rio de Janeiro, Brazil
Induced alkoxyresorufin-O-dealkylases in tilapias (Oreochromis niloticus) from Guandu river, Rio de Janeiro, Brazil
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DOI：
10.1016/j.chemosphere.2003.09.027
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发表时间：
2004-03-01
期刊：
影响因子：
8.8
通讯作者：
Paumgartten, FJR
中科院分区：
文献类型：
--
作者：
Parente, TEM;De-Oliveira, ACAX;Paumgartten, FJR
鱼类单加氧酶的活性已被广泛用作检测水体受细胞色素P450诱导剂污染的监测工具。在这项研究中，我们评估的活性乙氧基-（EROD），甲氧基（MROD）和戊氧基-（PROD）试卤灵-O-脱烷基酶的尼罗罗非鱼（Oreochromis niloticus）的肝脏收集在官渡河，在参考清洁网站（湖1）和其他两个采样点（湖2和3）在里约热内卢州，巴西。在肝S9组分中通过荧光法测定烷氧基试卤灵-O-脱烷基酶。官渡河罗非鱼的EROD、MROD和PROD活性分别为对照组的17.7倍、14.2倍和14.2倍。在3号湖的罗非鱼中也发现了EROD（5.0倍）和MROD（5.4倍）的适度增加。这些研究结果表明，官渡河流域，城市饮用水供应的主要来源，在里约热内卢，污染与CYP 1A诱导异生物质。此外，我们还发现EROD和MROD之间存在良好的线性关系，这一发现与O.尼罗河。(C)2003爱思唯尔有限公司。保留所有权利。
The activity of fish monooxygenases has been extensively used as a monitoring tool to detect contamination of water bodies by cytochrome P450-inducing agents. In this study we evaluated the activities of ethoxy- (EROD), methoxy(MROD) and pentoxy- (PROD) resorufin-O-dealkylases in the liver of Nile tilapias (Oreochromis niloticus) collected at the Guandu river, at a reference clean site (Lake 1) and at two other sampling sites (Lakes 2 and 3) in Rio de Janeiro state, Brazil. Alkoxyresorufin-O-dealkylases were measured fluorimetrically in the hepatic S9 fraction. EROD (17.7-fold), MROD (14.2-fold) as well as PROD activities were considerably higher in tilapias from Guandu river. A moderate increase of EROD (5.0-fold) and MROD (5.4-fold) was also found in tilapias from Lake 3. These findings suggest that Guandu river watershed, the main source of urban drinking water supply in Rio de Janeiro, is polluted with CYP1A-inducing xenobiotics. Furthermore, we also found a good linear relationship between EROD and MROD, a finding that agrees with the hypothesis that the two reactions are catalysed by the same CYP1A isoform in O. niloticus. (C) 2003 Elsevier Ltd. All rights reserved.