Radical Quenching of 1,1-Diphenyl-2-picrylhydrazyl: A Spectrometric Determination of Antioxidant Behavior
Radical Quenching of 1,1-Diphenyl-2-picrylhydrazyl: A Spectrometric Determination of Antioxidant Behavior
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1,1-二苯基-2-三硝基苯肼的自由基猝灭：抗氧化行为的光谱测定
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发表时间：
2008
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影响因子：
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通讯作者：
Sandi L. Schulien
中科院分区：
文献类型：
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作者：
J. Berger;Roshniben J. Rana;Hira Javeed;Iqra Javeed;Sandi L. Schulien
自由基化学是本科有机化学的一门基础学科。虽然有机教科书中有很好的描述，但实验室手册很少涉及这个主题，而是关注使用极性反应的合成。在此，我们描述了一种使用持久性自由基1,1-二苯基-2-吡啶酰肼（DPPH）的比色法测定一（3小时）实验室周期内常见抗氧化剂的抗氧化活性。虽然实验程序是为二年级的有机实验室环境设计的，但对实验或补充材料的微小修改将使其适合或多或少的高级课程。这个练习特别适合营养学或食品科学专业的学生。
Radical chemistry is a fundamental subject of undergraduate organic chemistry. While well described in organic textbooks, laboratory manuals rarely address this topic, focusing instead on syntheses using polar reactions. Herein, we describe a colorimetric assay using the persistent radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) to measure the antioxidant activity of common antioxidants in one (3 hour) lab period. While the laboratory procedures are designed for a second-year organic laboratory environment, minor modifications to either the experiment or the supplemental material would make this suitable for more or less advanced courses. This exercise is particularly appropriate for a course geared for nutrition or food science majors.