Molecular Characterization of a Novel Botulinum Neurotoxin Type H Gene
Molecular Characterization of a Novel Botulinum Neurotoxin Type H Gene
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DOI：
10.1093/infdis/jit450
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发表时间：
2014-01-15
影响因子：
6.4
通讯作者：
Arnon, Stephen S.
中科院分区：
文献类型：
--
作者：
Dover, Nir;Barash, Jason R.;Arnon, Stephen S.
我们对肉毒梭菌菌株 IBCA10-7060 Bh 型的 2 个肉毒毒素基因簇进行了测序。 bont/H 的序列与 A-G 型毒素的 7 个已知 bont 基因的序列有很大不同。编码肉毒杆菌毒素轻链的bont/H 5'三分之一末端与毒素类型A-G的轻链编码序列显着不同。编码肉毒杆菌毒素重链的bont/H的3'三分之二末端包含新的Hn易位结构域编码序列和非中和A型Hc结合结构域编码序列。 bont/H 是 orfX 毒素基因簇的一部分，该基因簇位于远离其他肉毒杆菌毒素基因簇所使用的染色体位点的独特染色体位点。 bont/B 序列与亚型bont/B2 相似，位于其ha 毒素基因簇内的oppA/brnQ 位点。我们的研究结果进一步证实肉毒梭菌 IBCA10-7060 产生新型 BoNT/H。
We sequenced the 2 botulinum toxin gene clusters of Clostridium botulinum strain IBCA10-7060 type Bh. The sequence of bont/H differed substantially from the sequences of the 7 known bont genes for toxin types A-G. The 5' one-third terminus of bont/H that codes for the botulinum toxin light chain differed markedly from the light chain coding sequences of toxin types A-G. The 3' two-thirds terminus of bont/H that codes for the botulinum toxin heavy chain contained a novel Hn translocation domain coding sequence and a nonneutralizing type A-like Hc binding domain coding sequence. bont/H was part of an orfX toxin gene cluster that was located at a unique chromosomal site distant from those used by other botulinum toxin gene clusters. The bont/B sequence was similar to that of subtype bont/B2 and was located within its ha toxin gene cluster at the oppA/brnQ site. Our findings further establish that C. botulinum IBCA10-7060 produces novel BoNT/H.