ROLE OF GRAFTED SKIN IN REGENERATION OF X-IRRADIATED AXOLOTL LIMBS
ROLE OF GRAFTED SKIN IN REGENERATION OF X-IRRADIATED AXOLOTL LIMBS
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DOI：
10.1016/0012-1606(77)90157-9
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发表时间：
1977-01-01
影响因子：
2.7
通讯作者：
NAMENWIRTH, M
中科院分区：
文献类型：
--
作者：
DUNIS, DA;NAMENWIRTH, M
对从肢体皮肤衍生的细胞进行肢体再生过程的跟踪，以确定这些细胞可能呈现的分化范围。将三倍体后肢或前肢皮肤移植到二倍体宿主蝾螈的裸露大腿上，该蝾螈的后肢和骨盆区域先前接受过 3000 R 的 X 射线照射。然后宿主的肢体通过移植物被截肢并允许再生。所有再生肢体均含有软骨、软骨膜、关节结缔组织、全身结缔组织、真皮和表皮，但只有10%的再生肢体含有肌肉。核仁数量列表显示，每个再生组织中的大多数细胞都源自移植皮肤。皮片移植的前肢或后肢起源与再生的手指数量之间存在很强的相关性。
The cells descended from limb skin were followed through the process of limb regeneration to determine what range of differentiations these cells may assume. Triploid hindlimb or forelimb skin was grafted to the denuded thighs of diploid host axolotls that had previously received 3000 R of X irradiation across both hindlimbs and the intervening pelvic area. The host limbs were then amputated through their grafts and permitted to regenerate. Cartilage, perichondrium, joint connective tissue, general connective tissue, dermis and epidermis were present in all the regenerated limbs, but only 10% of the regenerates contained muscle. Tabulation of nucleolar numbers showed that the majority of cells in each regenerated tissue originated from the grafted skin. A strong correlation was demonstrated between the forelimb or hindlimb origin of the skin grafts and the number of digits regenerated.