The effects of extracellular pH on bone resorption by avian osteoclasts in vitro
The effects of extracellular pH on bone resorption by avian osteoclasts in vitro
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体外pH值对禽类破骨细胞骨吸收的影响
DOI：
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发表时间：
1990
影响因子：
6.2
通讯作者：
J. Gallagher
中科院分区：
文献类型：
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作者：
C. A. Walsh;W. E. Dawson;M. Birch;J. Gallagher
此前已有报道称，低细胞外pH会刺激啮齿动物破骨细胞在体外挖掘吸收腔隙。在类似的体外测定中使用禽类骨细胞，我们证明破骨细胞活性在 pH 7.20-7.40 时最佳，并且在极端 pH 值（<7.10 和 >7.60）下受到抑制。在低 pH 值孵育的前 24 小时内，破骨细胞吸收可能会增加，但程度低于啮齿动物细胞报道的程度。然而，培养 24-30 小时后，骨吸收几乎没有或没有进一步增加，可能是由于低 pH 值直接或通过非破骨细胞骨细胞作用于破骨细胞的细胞毒性作用。之前的报告中，晶片预孵育 24 小时对骨吸收没有影响，与此相反，我们发现骨基质在 pH 6.50 下预孵育较长时间会增强随后在 pH 7.20 下的骨吸收。
It has previously been reported that low extracellular pH stimulates the excavation of resorption lacunae by rodent osteoclasts in vitro. Using avian bone cells in a similar in vitro assay we have demonstrated that osteoclast activity is optimal at pH 7.20–7.40 and is inhibited at extremes of pH (<7.10 and >7.60). Over the first 24 h of incubation at low pH there may be an increase in osteoclastic resorption but to a lesser extent than that reported for rodent cells. However, after 24–30 h in culture there is little or no further increase in bone resorption, presumably due to a cytotoxic effect of low pH acting either on the osteoclast directly or via nonosteoclastic bone cells. In contrast to a previous report, in which preincubation of wafers for 24 h had no effect on bone resorption, we found that preincubation of bone substrates at pH 6.50 for longer periods enhances subsequent resorption at pH 7.20.
影响因子：
15.9
作者：
TETI, A;BLAIR, HC;HRUSKA, KA
通讯作者：
HRUSKA, KA