A Selective Medium for Pyocyanin-dependent Fast Electrochemical Detection of Pseudomonas aeruginosa in Environmental Microbial Samples
A Selective Medium for Pyocyanin-dependent Fast Electrochemical Detection of Pseudomonas aeruginosa in Environmental Microbial Samples
复制标题
用于环境微生物样品中绿脓杆菌依赖性快速电化学检测铜绿假单胞菌的选择性介质
DOI：
10.20964/2018.04.20
复制
发表时间：
2018-04
影响因子：
1.5
通讯作者：
Polly H.M.Leung
中科院分区：
文献类型：
--
作者：
Shuihong Li;Qianqian Mou;Nuozhou Feng;Polly H.M.Leung
在这项工作中，选择性培养基绿脓杆菌依赖的定性检测铜绿假单胞菌的原微生物样品，采用电化学分析。为获得最佳的营养配方，研究比较了不同培养基（BYEB、LB、SDB、MHB、TSB和NB）、阳离子（K +、Na +、Ca 2+、Fe 3+和Mg 2+）和添加剂（1-萘胺、乙酰胺和青霉素钠）对绿脓菌素合成量的影响。结果表明，NB是绿脓菌素电化学检测的最佳介质，Mg 2+是绿脓菌素电化学检测的最佳辅助添加离子。1-与其他两种抗生素相比，当在培养基中提供以增强绿脓杆菌的存在时，萘胺被证明是最有效的支持抗生素试剂。结果表明，改良选择性培养基（ISM）能有效抑制铜绿假单胞菌以外的多种微生物的生长，并能显著提高含铜绿假单胞菌的环境样品中绿脓菌素的产量。本研究为复杂微生物样品中铜绿假单胞菌的电化学检测提供了一种简便、快速的方法。
In this work, a selective medium was developed for pyocyanina-dependent qualitative detection of Pseudomonas aeruginosa in raw microbial samples using electrochemical analysis. To obtain an optimum nutritive formulation, the effect of different media (BYEB, LB, SDB, MHB, TSB, and NB), positive ions (K + , Na + , Ca 2+ , Fe 3+ , and Mg 2+ ) and selected additives (1-naphthylamine, acetamide, and benzylpenicillin sodium) on the amount of synthesized pyocyanin were investigated and compared. NB was found to be the most favourable medium for the electrochemical detection of pyocyanin, and Mg 2+ was shown to be the most effective supporting additive ion for the detection. 1-Naphthylamine was shown to be the most effective supporting antibiotic reagent when supplied in the medium to enhancing the presence of pyocyanina compared to the other two antibiotics. The results indicated that the improved selective medium (ISM) can effectively inhibit/limit the growth of various microorganisms other than P. aeruginosa and obviously enhance the production of pyocyanin in environmental samples that contained P. aeruginosa . This study provided a method for simple and fast electrochemical detection of P. aeruginosa in complex microbial samples.