Regulation of histone acetyltransferase activity of p300 and PCAF by proto-oncogene protein DEK
Regulation of histone acetyltransferase activity of p300 and PCAF by proto-oncogene protein DEK
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DOI：
10.1016/j.febslet.2006.04.081
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发表时间：
2006-05-29
期刊：
影响因子：
3.5
通讯作者：
Seo, Sang-Beom
中科院分区：
文献类型：
--
作者：
Ko, Soo-Il;Lee, In-Seon;Seo, Sang-Beom
原癌基因蛋白DEK与急性髓细胞性白血病（AML）亚型相关的t（6;9）染色体易位有关，其导致DEK-CAN融合蛋白的形成。组蛋白乙酰化是一种重要的翻译后修饰，参与转录调控。在这项研究中，我们报告的酸性结构域包含蛋白DEK与组蛋白相互作用，并施加一个有效的抑制作用p300和PCAF介导的组蛋白乙酰转移酶的活性和转录。使用染色质免疫沉淀分析，我们已经证明，招募DEK适当的启动子诱导组蛋白H3和H4的低乙酰化染色质。总的来说，我们的数据说明了蛋白DEK在转录调控中发挥的重要调节作用，并表明转录调节酸性结构域区域可能在白血病发生中发挥作用。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The proto-oncogene protein DEK has been implicated in the t(6;9) chromosomal translocation associated with a subtype of acute myelogenous leukemia (AML), which results in the formation of a DEK-CAN fusion protein. Histone acetylation is an important post-translational modification which is involved in transcriptional regulation. In this study, we report that the acidic domain containing protein DEK interacts with histones and exerts a potent inhibitory effect on both p300 and PCAF-mediated histone acetyltransferase activity and transcription. Using chromatin immunoprecipitation assays, we have demonstrated that the recruitment of DEK to the appropriate promoter induces the histone H3 and H4 hypoacetylation of chromatin. Collectively, our data illustrate the important regulatory role played by protein DEK in transcriptional regulation, and suggest that transcription-regulating acidic domain regions may play a role in leukemogenesis. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.