Polyclonal B cell activators and in vitro induction of auto-antibody reactive with collagen.
Polyclonal B cell activators and in vitro induction of auto-antibody reactive with collagen.
复制标题
多克隆 B 细胞激活剂以及体外诱导与胶原蛋白发生反应的自身抗体。
DOI：
10.1111/j.1600-0765.1997.tb00938.x
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发表时间：
1997
影响因子：
3.5
通讯作者：
Tew,JG
中科院分区：
文献类型：
--
作者：
Hahn,CL;Schenkein,HA;Tew,JG
已知产生自身抗体的细胞存在于慢性发炎的牙周组织中。在慢性炎症部位，已知多克隆B细胞活化剂（PBA）在与外源抗原组合时表现出佐剂活性。这些结果促使检查PBA在引发对自身抗原（即I型胶原蛋白）的抗体应答中的作用。用大鼠Ⅰ型胶原、微生物PBA（LPS）或LPS+大鼠Ⅰ型胶原联合刺激大鼠淋巴细胞。使用ELISPOT测定法计数抗胶原抗体形成细胞（AFC）。单独的胶原或LPS引起很少的抗胶原AFC，但向胶原中添加LPS导致实质性的佐剂作用，并产生对胶原的最大反应。对短期免疫（加强后2 - 6周）、非免疫和长期免疫（加强后3 - 4个月）动物的抗胶原AFC进行了比较。结果表明，新免疫大鼠的细胞比其他两组更容易被激活。微生物PBA的辅助作用可能在抗胶原抗体产生中很重要，因此PBA在牙周袋中的定位可以解释为什么抗胶原AFCare仅限于慢性炎症牙周组织。
Cells producing autoantibodies are known to be present in chronically inflamed periodontal tissues. In sites of chronic inflammation, polyclonal B cell activators (PBA) are known to exhibit adjuvant activity when combined with foreign antigens. These results prompted an examination of PBA in eliciting an antibody response to an autoantigen (i.e. collagen type I). Rat lymphocytes were stimulated with rat collagen (type I), microbial PBA (LPS) or the combination of LPS plus rat collagenin vitro. Anti‐collagen antibody‐forming cells (AFC) were enumerated using anELISPOTassay. Collagen or LPS alone elicited few anti‐collagen AFC but the addition of LPS to collagen resulted in a substantial adjuvant effect and yielded maximal responses to collagen. Comparisons of anti‐collagenAFCfrom short‐term immunized (2‐6 wk after booster), non‐immunized and long‐term immunized (3‐4 months after booster) animals were performed. It revealed that cells from recently immunized rats werc significantly easier to activate than the other 2 groups. The adjuvant effect of microbialPBAmay be important in anti‐collagen antibody production and thus the localization of PBA in periodontal pockets may explain why anti‐ collagenAFCare restricted to the chronically inflamed periodontal tissues.