[Pharmacologic effects of biotin on epidermal cells].
[Pharmacologic effects of biotin on epidermal cells].
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[生物素对表皮细胞的药理作用]。
DOI：
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发表时间：
1991
影响因子：
0.7
通讯作者：
F. Althaus
中科院分区：
文献类型：
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作者：
A. Fritsche;G. Mathis;F. Althaus
动物体内生物素缺乏会引起皮肤及其附属物的病理变化，包括，例如，剥脱性皮炎、色素脱失和脱发。缺乏生物素的猪的蹄子软弱、易碎，而且经常坏死。这些变化在补充生物素后消失。补充生物素还能显著改善无明显生物素缺乏症的马、牛和猪的蹄质量。为了阐明这些作用的分子基础，利用电泳和免疫学技术研究了生物素对角质形成细胞系（Ha-CaT）细胞角蛋白表达的影响。培养液中1微米和100微米的药理生物素浓度引起细胞角蛋白的特异性增加，这通常是在体内表皮细胞终末分化时诱导的。细胞角蛋白的表达发生在独立分化的分层上皮不受影响。这些发现表明生物素直接刺激表皮细胞的分化。细胞培养中揭示的这一分子机制可以解释药理剂量生物素对农场动物蹄品质的治疗作用。
Biotin deficiency in animals causes pathological changes of the skin and its appendages including, for example, exfoliative dermatitis, depigmentation, and alopecia. The hooves of biotin-deficient swine are weak, brittle, and often necrotic. These changes disappear after dietary biotin supplementation. Biotin supplementation also noticeably improves the hoof quality of horses, cattle and swine having no apparent biotin deficiency. In order to elucidate the molecular basis of these effects, the influence of biotin on cytokeratin expression in a keratinocyte cell line (Ha-CaT) was investigated using electrophoretic and immunological techniques. Pharmacological biotin concentrations of 1 microM, and 100 microM in the culture medium caused a specific increase in cytokeratins, which are normally induced upon terminal differentiation of epidermal cells in vivo. The expression of cytokeratins occurring in stratified epithelia independent of differentiation were not affected. These findings show that biotin directly stimulates the differentiation of epidermal cells. Such a molecular mechanism revealed in cell culture could provide an explanation for the therapeutic effects of pharmacological doses of biotin on hoof quality in farm animals.