Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging
Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging
复制标题
猕猴白细胞、血浆和尿液中 DNA、RNA 及其代谢物的氧化损伤：尿液中的 8-氧代鸟苷是衰老的有用标志物
DOI：
10.3109/10715762.2012.689428
复制
发表时间：
2012-09-01
影响因子：
3.3
通讯作者：
Cai, Jian-Ping
中科院分区：
文献类型：
--
作者：
Shi, Fei;Nie, Ben;Cai, Jian-Ping
采用高效液相色谱-三重四极杆质谱法（LC-MS/MS）测定了猕猴白细胞、血浆和尿液中鸟苷的氧化形式。白细胞中分别来源于DNA和RNA的8-氧代-7，8-二氢脱氧鸟苷（8-氧代-dGsn）和8-氧代-7，8-二氢鸟苷（8-氧代Gsn）的量随着年龄的增长而增加。游离形式的氧化鸟苷的测量揭示了血浆和尿液中8-oxo-dGsn和8-oxoGsn的相似的年龄依赖性增加，其显示8-oxoGsn的量显著大于8-oxo-dGsn。尿液中的8-oxoGsn含量可能是评估衰老的有用生物标志物，因为与血浆相比，尿液中8-oxoGsn的年龄依赖性增加更明显，并且因为尿液样品很容易获得。
The levels of the oxidised forms of guanosine in leukocytes, plasma and urine of Macaca mulatta were determined using a sensitive method based on high-performance liquid chromatography-triple quadruple mass spectrometry (LC-MS/MS). The amounts of 8-oxo-7,8-dihydrodeoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosin (8-oxoGsn), derived from DNA and RNA, respectively, increased with age in leukocytes. The measurement of the free forms of oxidised guanosine revealed similar age-dependent increases of 8-oxo-dGsn and 8-oxoGsn in both plasma and urine, which showed considerably larger amounts of 8-oxoGsn than 8-oxo-dGsn. The 8-oxoGsn content of urine could be a useful biomarker for evaluating aging, as age-dependent increases of 8-oxoGsn are more evident in urine compared to plasma and because urine samples are readily available.