Distinct behaviors of neural stem and progenitor cells underlie cortical neurogenesis
Distinct behaviors of neural stem and progenitor cells underlie cortical neurogenesis
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DOI：
10.1002/cne.21669
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发表时间：
2008-05-01
影响因子：
2.5
通讯作者：
Kriegstein, Arnold R.
中科院分区：
文献类型：
--
作者：
Noctor, Stephen C.;Martinez-Cerdeno, Veronica;Kriegstein, Arnold R.
新皮质前体细胞经历对称和不对称分裂，同时产生大量不同的皮质细胞类型。在果蝇中，卵裂面的方向决定了遗传的命运决定因素和对称的新生子细胞在神经母细胞分裂。用于预测哺乳动物新皮层中的子细胞命运的一个模型也基于卵裂平面取向。预测具有相对于心室表面垂直（垂直）的分裂平面取向的前体细胞分裂是对称的，而预测具有平行于表面（水平）的分裂是不对称的神经原性分裂。然而，在脑室的卵裂平面方向的分析表明，预测的神经原性分裂的数量可能不足以产生大量的皮质神经元。为了了解与细胞分裂对称性相关的因素，我们通过实时成像、标记物分析和电生理记录原位检查了大鼠新皮质前体细胞。我们发现，卵裂面的方向是更密切相关的前体细胞类型比子细胞的命运，因为通常认为。VZ中的放射状胶质细胞主要在整个皮质发育过程中以垂直方向分裂，并根据发育阶段进行对称或不对称的自我更新分裂。相反，大多数中间祖细胞在室管膜下区水平方向分裂，产生对称的子细胞。我们提出了一个模型预测子细胞的命运，认为前体细胞类型，发展阶段，和平面分离的命运决定因素。
Neocortical precursor cells undergo symmetric and asymmetric divisions while producing large numbers of diverse cortical cell types. In Drosophila, cleavage plane orientation dictates the inheritance of fate-determinants and the symmetry of newborn daughter cells during neuroblast cell divisions. One model for predicting daughter cell fate in the mammalian neocortex is also based on cleavage plane orientation. Precursor cell divisions with a cleavage plane orientation that is perpendicular with respect to the ventricular surface (vertical) are predicted to be symmetric, while divisions with a cleavage plane orientation that is parallel to the surface (horizontal) are predicted to be asymmetric neurogenic divisions. However, analysis of cleavage plane orientation at the ventricle suggests that the number of predicted neurogenic divisions might be insufficient to produce large amounts of cortical neurons. To understand factors that correlate with the symmetry of cell divisions, we examined rat neocortical precursor cells in situ through real-time imaging, marker analysis, and electrophysiological recordings. We find that cleavage plane orientation is more closely associated with precursor cell type than with daughter cell fate, as commonly thought. Radial glia cells in the VZ primarily divide with a vertical orientation throughout cortical development and undergo symmetric or asymmetric self-renewing divisions depending on the stage of development. In contrast, most intermediate progenitor cells divide in the subventricular zone with a horizontal orientation and produce symmetric daughter cells. We propose a model for predicting daughter cell fate that considers precursor cell type, stage of development, and the planar segregation of fate determinants.