Electrical stimulation modulates fate determination of differentiating embryonic stem cells
Electrical stimulation modulates fate determination of differentiating embryonic stem cells
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DOI：
10.1634/stemcells.2006-0011
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发表时间：
2007-03-01
期刊：
影响因子：
5.2
通讯作者：
Kondo, Takashi
中科院分区：
文献类型：
--
作者：
Yamada, Masahisa;Tanemura, Kentaro;Kondo, Takashi
在分化过程中对细胞命运调控的清楚理解是成功地将干细胞用于治疗应用的关键。在这里，我们报告说，温和的电刺激强烈影响胚胎干细胞承担神经元的命运。虽然所得到的神经元细胞在培养中没有表现出特异性终末分化的迹象，但它们在体内表现出分化为各种类型神经元的潜力，并且在成年小鼠中，作为神经元细胞对受损的脊髓有贡献。钙离子内流的诱导在该分化系统中是重要的。这一现象为理解细胞分化和早期发育的新机制提供了可能性，也许更重要的是，它表明了医学背景下治疗的可能性。
A clear understanding of cell fate regulation during differentiation is key in successfully using stem cells for therapeutic applications. Here, we report that mild electrical stimulation strongly influences embryonic stem cells to assume a neuronal fate. Although the resulting neuronal cells showed no sign of specific terminal differentiation in culture, they showed potential to differentiate into various types of neurons in vivo, and, in adult mice, contributed to the injured spinal cord as neuronal cells. Induction of calcium ion influx is significant in this differentiation system. This phenomenon opens up possibilities for understanding novel mechanisms underlying cellular differentiation and early development, and, perhaps more importantly, suggests possibilities for treatments in medical contexts.