Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei
Germ line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): Evidence for vasa localization at cleavage furrows in euteleostei
复制标题
vasa 同源基因在大菱鲆（大菱鲆）种系特异性表达：vasa 定位于真骨卵裂沟的证据
DOI：
10.1002/mrd.22120
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发表时间：
2012-11-01
影响因子：
2.5
通讯作者：
Li, Jun
中科院分区：
文献类型：
--
作者：
Lin, Fan;Xu, Shihong;Li, Jun
胚胎早期发育过程中原始生殖细胞的特化是生殖和发育过程中的一个重要生物学问题。然而，人们对海洋经济型鱼类知之甚少。VASA是种质的组成部分，是硬骨鱼中记录最多的生殖细胞标记物。本研究从海洋真骨目大比目鱼中分离到一个全长VasA基因(SMVAS)，并通过RT-PCR和原位杂交研究其在胚胎发生和配子发生过程中的表达模式，以鉴定该物种的生殖细胞谱系。其推导的氨基酸序列具有典型的Vasa蛋白特征，与青冈鱼(76.9%)和斑马鱼(68.5%)的Vasa同源物有较高的同源性。SMVAS转录本仅在睾丸和卵巢的生殖细胞中检测到，并在卵子发生过程中表现出有趣的动态定位模式。Smvas在胚胎发生过程中的分布模式与斑马鱼(Osteriophysans)的分布模式非常相似，而不是青海斑马鱼(Eutelestei)的。因此，本研究分离到的SMVAS基因是大比目鱼生殖细胞特异的分子标记。此外，我们还假设Eutelebone可以通过一种特殊的方式定位Vasa mRNA。这些结果不仅为大比目鱼生殖细胞的操作提供了便利，也加深了我们对硬骨鱼生殖系发育和Vasa定位进化的理解。摩尔。是Reprod。戴夫。2012年，79：803813。(C)2012年威利期刊公司。
Specification of primordial germ cells during early embryogenesis is a critical biological issue in reproduction and development. Yet, little is known in marine economic fish species. Vasa, a component of germ plasm, is the most-documented germ cell marker in teleosts. We isolated a full-length vasa cDNA (Smvas) from turbot (Scophthalmus maximus), a marine Euteleostei species, and investigated its expression patterns by RT-PCR and in situ hybridization during embryogenesis and gametogenesis to identify the germ cell lineage in this species. The deduced amino acid sequence of the isolated cDNA shared typical characteristics of Vasa protein and high identity to Vasa homologues in medaka (76.9%) and zebrafish (68.5%). The Smvas transcripts were exclusively detected in germ cells of testis and ovary, and exhibited an interesting dynamic localization pattern during oogenesis. The distribution pattern of Smvas during embyogenesis in this Euteleostei closely resembled the pattern observed in zebrafish (belonging to Osteriophysans) rather than medaka (belonging to Euteleostei). Thus, it is concluded that Smvas isolated in this study is a germ cell specific molecular marker in turbot. Furthermore, we hypothesize that Euteleostei could localize vasa mRNA by a special mode. The results not only facilitate the germ cell manipulation of the turbot, but also improve our understanding of germline development and evolution of vasa localization in teleost. Mol. Reprod. Dev. 79: 803813, 2012. (C) 2012 Wiley Periodicals, Inc.