Isolation and characterization of Escherichia coli O157 from retail beef and bovine feces in Thailand.
Isolation and characterization of Escherichia coli O157 from retail beef and bovine feces in Thailand.
复制标题
从泰国零售牛肉和牛粪便中分离和鉴定大肠杆菌 O157。
DOI：
10.1111/j.1574-6968.2000.tb08919.x
复制
发表时间：
2000
影响因子：
2.1
通讯作者：
M. Nishibuchi
中科院分区：
文献类型：
--
作者：
V. Vuddhakul;Nuanjira Patararungrong;Punnee Pungrasamee;S. Jitsurong;T. Morigaki;Norio Asai;M. Nishibuchi
大肠杆菌O 157脂多糖抗体检测健康人的血清中更频繁地在泰国南部比在日本。结果提示泰国人可能接触过大肠杆菌。coli O157。E.从泰国南部采集的95份零售牛肉样品中的4份和55份牛粪样品中的1份中分离到O 157：H7或O 157：H（-）大肠杆菌。5株中有4株单独携带stx（2）基因或同时携带stx（1）基因。通过任意引物PCR方法显示菌株在遗传上不同。
Antibody to Escherichia coli O157 lipopolysaccharide was detected in the sera of healthy individuals more frequently in Southern Thailand than in Japan. The result suggested possible exposure of Thai people to E. coli O157. E. coli O157:H7 or O157:H(-) was isolated from four of 95 retail beef and one of 55 bovine feces samples collected in Southern Thailand by enrichment culture followed by immunomagnetic bead separation. Four of the five strains carried the stx(2) gene alone or in combination with the stx(1) gene. The strains were shown to be genetically distinct by an arbitrarily primed PCR method.