LASER TEMPERATURE-JUMP, SPECTROSCOPIC, AND THERMODYNAMIC STUDY OF SALT EFFECTS ON DUPLEX FORMATION BY DGCATGC
LASER TEMPERATURE-JUMP, SPECTROSCOPIC, AND THERMODYNAMIC STUDY OF SALT EFFECTS ON DUPLEX FORMATION BY DGCATGC
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DOI：
10.1021/bi00436a025
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发表时间：
1989-05-16
期刊：
影响因子：
2.9
通讯作者：
TURNER, DH
中科院分区：
文献类型：
--
作者：
WILLIAMS, AP;LONGFELLOW, CE;TURNER, DH
Salt effects of duplex formation by dGCATGC have been studied with spectroscopic, thermodynamic, and kinetic methods. Circular dichroism spectra indicate different salt conditions have little effect on the structures of the duplex and single strand. NMR chemical shifts indicate the structure of the duplex in 1 M NaCl is similar to that of the B-form determined previously in 0.5 M KCl [Nilges, M., Clore, G. M., Gronenborn, A. M., Brunger, A. T., Karplus, M., and Nilsson, L. (1987) Biochemistry 26, 3718-3733]. Optical melting experiments indicate the effect of Na+ concentration on melting temperature is similar to that expected for a polynucleotide with the same GC content. Laser temperature-jump experiments indicate the effect of Na+ concentration on the rate of duplex formation is much less than is observed for polynucleotides. The observations are consistent with expectations based on a counterion condensation model. This is surprising for a duplex with only 10 phosphates.
