The effect of okadaic acid on meiotic maturation of canine oocytes of different size
The effect of okadaic acid on meiotic maturation of canine oocytes of different size
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DOI：
10.1016/j.theriogenology.2011.07.013
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发表时间：
2012-01-01
期刊：
影响因子：
2.8
通讯作者：
Bogliolo, Luisa
中科院分区：
文献类型：
--
作者：
Ariu, Federica;Fois, Stefano;Bogliolo, Luisa
本研究旨在探讨一种丝氨酸/苏氨酸1和2A磷酸酶的有效抑制剂冈地酸（OA）对不同直径犬卵母细胞减数分裂恢复和进行的影响。采集不同发情期母犬卵巢中的卵丘-卵母细胞复合体。在实验1中，为了确定OA的最佳浓度（0.5或2 μ M），将卵母细胞在补充有20%SCE的TCM 199中预孵育1、3和20小时，然后在不含OA的相同培养基中培养。在实验2中，根据卵母细胞的直径将所选择的卵母细胞分为三组：120 μ m，并在0.5 μ M的OA中预孵育1小时。如前所述体外培养卵母细胞。在实验1中，0.5 μ M处理1 h后，72 h的卵母细胞达到MII期的比例显著高于120 μ M处理组（30.8%，P < 0.05），120 μ M处理组（51.3%，P < 0.05），120 μ M处理组（51.0%，P < 0
The present study was conducted to determine the effect of okadic acid (OA), a potent inhibitor of seronine/treonine 1 and 2A phosphatase, on meiotic resumption and progression in canine oocytes with different diameters. Cumulus-oocyte complexes were collected from ovaries of bitches at different oestrous phases. In Experiment 1, to determine the optimal concentration of OA (0.5 or 2 mu M), the oocytes were pre-incubated for 1, 3, and 20 h in TCM 199 supplemented with 20% SCE and thereafter cultured in the same medium without OA. In Experiment 2, the selected oocytes were divided into three groups according to their diameter: 120 mu m, and pre-incubated in OA 0.5 mu M for 1 h. Oocytes were cultured in vitro as previously described. After 72 h of IVM, in Experiment 1, significantly more oocytes reached MII stage with 0.5 mu M for 1 h (30.8% P120 mu m groups (P120 mu m pre-incubated with OA progressed to MII (51.3% P