Roles of a membrane-localized beta subunit in the formation and targeting of functional L-type Ca2+ channels.
Roles of a membrane-localized beta subunit in the formation and targeting of functional L-type Ca2+ channels.
复制标题
膜定位 β 亚基在功能性 L 型 Ca2 通道的形成和靶向中的作用。
DOI：
10.1074/jbc.270.50.30036
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hosey,MM
中科院分区：
文献类型：
--
作者：
Chien,AJ;Zhao,X;Shirokov,RE;Puri,TS;Chang,CF;Sun,D;Rios,E;Hosey,MM
We report several unexpected findings that provide novel insights into the properties and interactions of the α1and β subunits of dihydropyridine-sensitive L-type channels. First, the β2asubunit was expressed as multiple species of 68-72 kDa; the 70-72-kDa species arose from post-translational modification. Second, cell fractionation and immunocytochemical studies indicated that the hydrophilic β2asubunit, when expressed alone, was membrane-localized. Third, the β2asubunit increased the membrane localization of the α1subunit and the number of cells expressing L-type Ca2+currents, without affecting the total amount of the expressed α1Csubunit. Expression of maximal currents in α1C/β2aco-transfected cells paralleled the time course of expression of the β subunit. Taken together, these results suggest that the β subunit plays multiple roles in the formation, stabilization, targeting, and modulation of L-type channels.
