Multiple gene deletion in Cryptococcus neoformans using the Cre-lox system.
Multiple gene deletion in Cryptococcus neoformans using the Cre-lox system.
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DOI：
10.1007/978-1-61779-539-8_6
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
Lodge, Jennifer K
中科院分区：
文献类型：
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作者：
Baker, Lorina G;Lodge, Jennifer K
反向遗传学通常用于鉴定和表征参与各种细胞过程的基因。有一组有限的阳性选择标记可用于在新生隐球菌中进行基因缺失或其他遗传操作。在这里，我们描述了噬菌体P1 Cre-loxP系统在C. neoformans，及其在遗传霉素药物标记物的切除和再利用中的应用。该工具将允许研究人员在同一菌株中进行多个连续的基因缺失，这将有助于多基因家族的分析。
Reverse genetics is commonly used to identify and characterize genes involved in a variety of cellular processes. There is a limited set of positive selectable markers available for use in making gene deletions or other genetic manipulations in Cryptococcus neoformans. Here, we describe the adaptation of the Bacteriophage P1 Cre-loxP system for use in C. neoformans, and its application in the excision and reuse of the geneticin drug marker. This tool will allow investigators to make multiple, sequential gene deletions in the same strain, which should facilitate the analysis of multigene families.