CONTENT OF MITOCHONDRIAL-DNA AND OF 3 MITOCHONDRIAL RNAS IN DEVELOPING AND ADULT-RAT CEREBELLUM
CONTENT OF MITOCHONDRIAL-DNA AND OF 3 MITOCHONDRIAL RNAS IN DEVELOPING AND ADULT-RAT CEREBELLUM
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DOI：
10.1111/j.1471-4159.1989.tb02518.x
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发表时间：
1989-03-01
影响因子：
4.7
通讯作者：
GADALETA, MN
中科院分区：
文献类型：
--
作者：
RENIS, M;CANTATORE, P;GADALETA, MN
DNA和16 S rRNA以及两种mRNA的含量，即，已经测定了发育和成年大鼠小脑的游离（非突触）线粒体中细胞色素c氧化酶亚基I的mRNA和NADH脱氢酶（ND 4）的一个亚基的mRNA。在出生后的发育过程中，游离（非突触）线粒体的DNA含量从1至30日龄增加10倍，而在成年大鼠中，与30日龄大鼠相比，约为60%。研究的每种RNA的总含量也在发育过程中增加。然而，当每种RNA的含量在每个mtDNA分子中表达时，rRNA和rRNA表现不同; 16 S rRNA水平在发育过程中不发生变化，与成年大鼠没有显著差异，而mRNA水平在发育过程中高于成年大鼠，并随年龄变化。这些结果进行了讨论，在大鼠小脑发育过程中的线粒体生物合成和线粒体DNA转录过程的调节。
The content of DNA and of 16S rRNA and of two mRNAs, i.e., the mRNA for the cytochrome c oxidase subunit I and the mRNA for one subunit of the NADH dehydrogenase (ND4), in free (nonsynaptic) mitochondria of developing and adult rat cerebellum has been determined. During postnatal development, DNA content of free (nonsynaptic) mitochondria increases 10 times from 1 to 30 days of age whereas, in adult rats, it is about 60% compared to that found in 30-day-old rats. The total content of each RNA species studied also increases during development. However, when the content of each RNA is expressed per mtDNA molecule, rRNAs and rRNAs behave differently; 16S rRNA level does not change during development and it is not significantly different from that of the adult rat, whereas the level of mRNAs is higher during development than in the adult rat and changes with age. These results are discussed in light of mitochondrial biogenesis in rat cerebellum during development and of the regulation of the mitochondrial DNA transcription process.