Mutagenesis of GmFT2a and GmFT5a mediated by CRISPR/Cas9 contributes for expanding the regional adaptability of soybean
Mutagenesis of GmFT2a and GmFT5a mediated by CRISPR/Cas9 contributes for expanding the regional adaptability of soybean
复制标题
DOI：
10.1111/pbi.13199
复制
发表时间：
2019-07-05
影响因子：
13.8
通讯作者：
Hou, Wensheng
中科院分区：
文献类型：
--
作者：
Cai, Yupeng;Wang, Liwei;Hou, Wensheng
开花时间是大豆（Glycine max）能否成功适应不同纬度和耕作制度的关键农艺性状。GmFT2a和GmFT5a被广泛鉴定为大豆开花激活因子和整合因子。在此基础上，我们发现了两个分别包含GmFT2a和GmFT5a的数量性状位点（qtl）区域，它们在不同光周期下对开花的遗传效应不同。我们分析了过表达GmFT2a或GmFT5a、ft2a突变体、ft5a突变体和ft2aft5a双突变体的转基因植株在长日照（LD）和短日照（SD）条件下的开花时间。我们证实GmFT2a和GmFT5a不是冗余的，它们共同调节开花时间，且在SD条件下GmFT2a的作用比GmFT5a更突出，而在LD条件下GmFT5a的作用比GmFT2a更显著。大豆适应高纬度地区所必需的基因是GmFT5a，而不是GmFT2a。双突变体ft2aft5a在SD条件下开花晚31.3天，单株荚果和种子数量显著高于野生型。我们推测这些突变体在热带地区可能具有巨大的产量潜力。此外，我们还对202份大豆材料中这两个位点的序列进行了分析，并对其主要单倍型的开花表型、地理分布和成熟群体进行了研究。这些结果将有助于大豆育种和区域适应性。
Flowering time is a key agronomic trait that directly influences the successful adaptation of soybean (Glycine max) to diverse latitudes and farming systems. GmFT2a and GmFT5a have been extensively identified as flowering activators and integrators in soybean. Here, we identified two quantitative trait loci (QTLs) regions harbouring GmFT2a and GmFT5a, respectively, associated with different genetic effects on flowering under different photoperiods. We analysed the flowering time of transgenic plants overexpressing GmFT2a or GmFT5a, ft2a mutants, ft5a mutants and ft2aft5a double mutants under long-day (LD) and short-day (SD) conditions. We confirmed that GmFT2a and GmFT5a are not redundant, they collectively regulate flowering time, and the effect of GmFT2a is more prominent than that of GmFT5a under SD conditions whereas GmFT5a has more significant effects than GmFT2a under LD conditions. GmFT5a, not GmFT2a, was essential for soybean to adapt to high latitude regions. The ft2aft5a double mutants showed late flowering by about 31.3 days under SD conditions and produced significantly increased numbers of pods and seeds per plant compared to the wild type. We speculate that these mutants may have enormous yield potential for the tropics. In addition, we examined the sequences of these two loci in 202 soybean accessions and investigated the flowering phenotypes, geographical distributions and maturity groups within major haplotypes. These results will contribute to soybean breeding and regional adaptability.