Granulocyte/macrophage colony-stimulating factor induces interleukin 1 production by human polymorphonuclear neutrophils.
Granulocyte/macrophage colony-stimulating factor induces interleukin 1 production by human polymorphonuclear neutrophils.
复制标题
粒细胞/巨噬细胞集落刺激因子诱导人多形核中性粒细胞产生白细胞介素 1。
DOI：
10.4049/jimmunol.140.3.837
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发表时间：
1988
影响因子：
4.4
通讯作者：
F. Herrmann
中科院分区：
文献类型：
--
作者：
A. Lindemann;D. Riedel;W. Oster;S. Meuer;D. Blohm;R. Mertelsmann;F. Herrmann
本研究的目的是确定是否人类多形核中性粒细胞（PMN），这与巨噬细胞共享一个共同的细胞谱系，可以产生因子，如IL-1。我们通过北方印迹分析和生物测定表明，PMN可以被诱导积累IL-1 α和IL-1 β特异性mRNA，其大小与活化的人巨噬细胞合成的IL-1 mRNA大小无区别，因此在其培养上清液中释放IL-1样活性，其可以被IL-1的mAb中和。粒细胞-巨噬细胞集落刺激因子被鉴定为PMN-IL-1的主要生理诱导剂。
The purpose of this study was to determine whether human polymorphonuclear neutrophils (PMN), which share a common cell lineage with macrophages, could produce factors such as IL-1. We show by Northern blot analysis and bioassays that PMN can be induced to accumulate mRNA specific for IL-1 alpha and IL-1 beta indistinguishable in size from IL-1 mRNA synthesized by activated human macrophages and consequently to release IL-1-like activity in their culture supernatants, that could be neutralized by a mAb to IL-1. The granulocyte-macrophage colony-stimulating factor was identified as a major physiologic inducer for PMN-IL-1.