The effect of zoledronate during bone healing.
The effect of zoledronate during bone healing.
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DOI：
10.1007/s10195-010-0083-1
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发表时间：
2010-03
影响因子：
2.8
通讯作者：
Guarniero, Roberto
中科院分区：
文献类型：
--
作者：
Matos, Marcos Almeida;Tannuri, Uenis;Guarniero, Roberto
双膦酸盐已成为多种骨骼疾病的首选治疗方法，其中过度的破骨细胞活动是一种重要的病理特征。然而，抑制破骨细胞活性可能会抑制骨愈合或修复过程中的重塑。本研究的目的是观察唑来膦酸盐(最有效的双磷酸盐)在骨愈合生物过程中的作用。将30只雄性幼兔随机分为对照组和试验组，每组15只。两组均接受腓骨截骨术。仅在试验组给予单剂唑来磷酸钠。在1、2和4周后，两组动物都被安乐死，并对截骨部位进行组织形态计量学评估。相关参数包括组织体积(TV)、骨小梁体积分数(BV/TV)、编织骨体积分数(WOV/TV)、骨膜纤维体积分数(FBV/TV)和髓质纤维体积(MAV/TV)。愈合第一周骨痂面积较小(实验组)，骨膜纤维化程度较轻。2周时两组均可见大量编织骨，骨膜纤维化明显减轻。对照组的骨小梁也有明显增加。第4周实验组编织骨和松质骨量增加，两组髓质纤维化增加，而实验组骨膜纤维化持续明显减轻。唑来膦酸盐不会阻止骨愈合。然而，唑来膦酸盐的作用特点是对初级骨生成的刺激加剧，可能抑制重塑，导致骨小梁保留。
Bisphosphonates have become the treatment of choice for a variety of bone diseases in which excessive osteoclastic activity is an important pathologic feature. However, inhibition of osteoclastic activity could lead to inhibition of remodeling during bone healing or repair. The objective of this study is to investigate the effect of zoledronate (the most potent bisphosphonate) in the biological process of bone healing. Thirty immature male rabbits were divided into two groups (control and experimental) of 15 animals each. Both groups were submitted to fibular osteotomy. Only in the experimental group a single dose of zoledronate was administered. After 1, 2, and 4 weeks, animals of both groups were euthanized and the osteotomy site was histomorphometrically evaluated. The associated parameters analyzed were tissue volume (TV), fractional trabecular bone volume (BV/TV), fractional woven bone volume (WoV/TV), fractional periosteal fibrous volume (FbV/TV), and medullary fibrous volume (MaV/TV). The first week of healing was characterized by small callus area (experimental group) and less periosteal fibrosis. The second week was characterized by a large quantity of woven bone and marked decrease in periosteal fibrosis in the two groups. In the control group there was also a significant increase in trabecular bone. The fourth week was characterized by increased amount of woven bone and trabecular bone in the experimental group; there was increased medullary fibrosis in the two groups, while there continued to be significantly less periosteal fibrosis in the experimental group. Zoledronate does not prevent bone healing. However, the effect of zoledronate was characterized by accentuated stimulation of primary bone production and probably inhibition of remodeling, leading to retention of trabecular bone.