Separation of furosemide, phenylbutazone and oxyphenbutazone in plasma by direct injection onto internal surface reversed-phase columns with systematic optimization of selectivity.
Separation of furosemide, phenylbutazone and oxyphenbutazone in plasma by direct injection onto internal surface reversed-phase columns with systematic optimization of selectivity.
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通过直接注射到内表面反相柱上并系统优化选择性来分离血浆中的呋塞米、保泰松和羟保泰松。
DOI：
10.1016/s0021-9673(00)94862-3
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Rateike,JD
中科院分区：
文献类型：
--
作者：
Pinkerton,TC;Perry,JA;Rateike,JD
ISRP概念基于尺寸排阻和内表面分区。ISRP支持物的中值孔径为52 A（参考文献10），因此将蛋白质限制在间隙空间中。球形二氧化硅载体的外表面通过甘油基丙基键合相对蛋白质不具有吸附性，因此蛋白质在间隙空隙体积中聚集。另一方面，药物和代谢物穿透包装并与甘氨酸-苯丙氨酸-苯丙氨酸分配相相互作用（图2）。在填充之前，通过酶裂解从外表面除去苯丙氨酸部分，因此载体的外表面保持不吸附蛋白质。在开发HPLC分离中，通常的做法是基于分析物分子结构和对所用键合相的保留特性的直观认识来选择初始移动的相条件。因此，第一次分离将产生重叠峰。然后，通过反复试验或系统地优化容量因子、选择性和效率来提高分辨率。
The ISRP concept is based upon size exclusion and internal surface partitioning. The ISRP supports have a median pore diameter of 52 A (ref. lo), thus confining proteins to the interstitial space. The external surfaces of the spherical silica supports are rendered non-adsorptive to the proteins via a glycerylpropyl-bonded phase, so proteins elute in the interstitial void volume. Drugs and metabolites, on the other hand, penetrate the packing and interact with a glycine-phenylalanine-phenylalanine partitioning phase (Fig. 2). Prior to packing, the phenylalanine moities are removed from the external surface by enzyme cleavage, so the external surface of the supports remains non-adsorptive to protein@.In developing an HPLC separation it is common practice to select an initial mobile phase condition based upon the analyte molecular structure and an intuitive knowledge of the retention characteristics of the bonded phase in use. Invariably the first separation will yield overlapping peaks. One then proceeds to improve resolution by trial and error or by systematically optimizing capacity factors, selectivity, and efficiency.